. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MERCAPTOETHANOL INDUCED DELAY 341 containing either ME or puromycin. After 5 minutes, 1 /zc/ml 3H-leucine (5.0 c/mmole, New England Nuclear) was added. The reaction was terminated by adding an equal volume of 0r/c trichloroacetic acid (TCA) and the samples 70 50 (D C £ 30 10. 10 30 50 TAI in minutes FIGURE 1. Delay-ME exposure diagram of Lytechinus eggs. Eggs were fertilized, and treated by ME for 15 minutes, initiated at different times after insemination. Lengths of each vertical line indicate the first cleavage period of
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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MERCAPTOETHANOL INDUCED DELAY 341 containing either ME or puromycin. After 5 minutes, 1 /zc/ml 3H-leucine (5.0 c/mmole, New England Nuclear) was added. The reaction was terminated by adding an equal volume of 0r/c trichloroacetic acid (TCA) and the samples 70 50 (D C £ 30 10. 10 30 50 TAI in minutes FIGURE 1. Delay-ME exposure diagram of Lytechinus eggs. Eggs were fertilized, and treated by ME for 15 minutes, initiated at different times after insemination. Lengths of each vertical line indicate the first cleavage period of Lytechinus eggs. Duration of the ME treatment is indicated by the solid line. Numbers associated with each vertical line indicate the recovery period. The mitotic stages of the control eggs are indicated on the right side of the graph. Abbreviations used are: TAI = time after insemination; m = multiple division in some eggs ; d = time of control cleavage ; mp = metaphase ; nm = disappearance of the nuclear membrane ; dc = centriole separation ; fp = fusion of pronuclei; excess delay = cleav- age period of ME treated eggs — cleavage period of control egg — duration of the ME treatment. were heated for 15 minutes at 90° C. An excessive amount of non-radioactive leucine was added and the samples were kept overnight in the cold room. The samples were filtered through glass fiber filters, washed with 70% ethanol, dried, placed in vials containing toluene and proper fluors and the radioactivity was counted using a Nuclear Chicago Scintillation Counter.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred C