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Bacteriology tubes Stock Photos and Images
Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . inih jiijx-this will ijive 1200 revolutionsa minute; .^o |x>unds will give1400; is |x>unds. 1500. Athou.sani) revolutions a min-ute are sufficient for urinesedimentation. Tubes . andM IimI.I ;o. . rarh. 936 GEXERAL LABORATORY TECHXIC. Examine with i-inch or ^-inch objective and make drawings of- Hlood-casts, Epithelial casts, Granular casts, Waxy casts, Fatty casts, Hyaline casts, Pus-casts, Bacterial casts, Cylindroids, False casts, composed of Piratesor crystals. Casts from s Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/manual-of-pathology-including-bacteriology-the-technic-of-postmortems-and-methods-of-pathologic-research-inih-jiijx-this-will-ijive-1200-revolutionsa-minute-o-xgtunds-will-give1400-is-xgtunds-1500-athousani-revolutions-a-min-ute-are-sufficient-for-urinesedimentation-tubes-andm-iimii-o-rarh-936-gexeral-laboratory-techxic-examine-with-i-inch-or-inch-objective-and-make-drawings-of-hlood-casts-epithelial-casts-granular-casts-waxy-casts-fatty-casts-hyaline-casts-pus-casts-bacterial-casts-cylindroids-false-casts-composed-of-piratesor-crystals-casts-from-s-image338392891.htmlRM2AJF3GB–Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . inih jiijx-this will ijive 1200 revolutionsa minute; .^o |x>unds will give1400; is |x>unds. 1500. Athou.sani) revolutions a min-ute are sufficient for urinesedimentation. Tubes . andM IimI.I ;o. . rarh. 936 GEXERAL LABORATORY TECHXIC. Examine with i-inch or ^-inch objective and make drawings of- Hlood-casts, Epithelial casts, Granular casts, Waxy casts, Fatty casts, Hyaline casts, Pus-casts, Bacterial casts, Cylindroids, False casts, composed of Piratesor crystals. Casts from s
. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. Fig. 190. Pkysaritm Uiicophaatm,Vi. a spor- angium seen from without, b sporangium divided in ludf and the frame-worlc of the capillitium exposed by removal of the spores. Magn. 25 times. Pig. 191. Physarum Uutophaeum, Fr. Piece of the wall of a sporangium with tubes of the capillitium attached and spread out in water; a points of attachment of the tubes of the capillitium; ^ calcium carbonate-vesicles; to the right on the margin a calcium carbonate- vesicle on the wa Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/comparative-morphology-and-biology-of-the-fungi-mycetozoa-and-bacteria-plant-morphology-fungi-myxomycetes-bacteriology-fig-190-pkysaritm-uiicophaatmvi-a-spor-angium-seen-from-without-b-sporangium-divided-in-ludf-and-the-frame-worlc-of-the-capillitium-exposed-by-removal-of-the-spores-magn-25-times-pig-191-physarum-uutophaeum-fr-piece-of-the-wall-of-a-sporangium-with-tubes-of-the-capillitium-attached-and-spread-out-in-water-a-points-of-attachment-of-the-tubes-of-the-capillitium-calcium-carbonate-vesicles-to-the-right-on-the-margin-a-calcium-carbonate-vesicle-on-the-wa-image216392855.htmlRMPG1F87–. Comparative morphology and biology of the fungi, mycetozoa and bacteria . Plant morphology; Fungi; Myxomycetes; Bacteriology. Fig. 190. Pkysaritm Uiicophaatm,Vi. a spor- angium seen from without, b sporangium divided in ludf and the frame-worlc of the capillitium exposed by removal of the spores. Magn. 25 times. Pig. 191. Physarum Uutophaeum, Fr. Piece of the wall of a sporangium with tubes of the capillitium attached and spread out in water; a points of attachment of the tubes of the capillitium; ^ calcium carbonate-vesicles; to the right on the margin a calcium carbonate- vesicle on the wa
. The principles of bacteriology: a practical manual for students and physicians. ated from the direc-tion given by v. Esmarch for this part of his method,and instead of rolling the tubes under ice-water, we rollthem upon a block of ice (Fig. 29), after the methoddevised by Booker in 1887 in the Pathological Labora-tory of the Johns Hopkins University. In this methoda small block of ice only is needed. It is levelledand held in position by being placed upon a towel ina dish. A horizontal groove is melted in the uppersurface of the ice with a test-tube of hot water. Thetubes to be rolled are th Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/the-principles-of-bacteriology-a-practical-manual-for-students-and-physicians-ated-from-the-direc-tion-given-by-v-esmarch-for-this-part-of-his-methodand-instead-of-rolling-the-tubes-under-ice-water-we-rollthem-upon-a-block-of-ice-fig-29-after-the-methoddevised-by-booker-in-1887-in-the-pathological-labora-tory-of-the-johns-hopkins-university-in-this-methoda-small-block-of-ice-only-is-needed-it-is-levelledand-held-in-position-by-being-placed-upon-a-towel-ina-dish-a-horizontal-groove-is-melted-in-the-uppersurface-of-the-ice-with-a-test-tube-of-hot-water-thetubes-to-be-rolled-are-th-image336995461.htmlRM2AG7D45–. The principles of bacteriology: a practical manual for students and physicians. ated from the direc-tion given by v. Esmarch for this part of his method,and instead of rolling the tubes under ice-water, we rollthem upon a block of ice (Fig. 29), after the methoddevised by Booker in 1887 in the Pathological Labora-tory of the Johns Hopkins University. In this methoda small block of ice only is needed. It is levelledand held in position by being placed upon a towel ina dish. A horizontal groove is melted in the uppersurface of the ice with a test-tube of hot water. Thetubes to be rolled are th
. Laboratory work in bacteriology. Bacteriology. fi^ A^- rOP OF /IVKSIf CPOSZ-SSCrioN. Fig. 38. Murrill's gas-pressure regulator. U tube containing water. The cylinder may be floated in water, but, inasmuch as this is liable to evaporate, it is better to use liquid paraffin. The apparatus is provided with two outflow tubes, which are to be connected with thermo-regulators. It is of convenient size, being only 6 1 Journ. of Applied Microscopy 1, p. 92, 1898. Centralblatt ffir Bakteriologie 23, p. 1056,1898.. Please note that these images are extracted from scanned page images that may have been Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/laboratory-work-in-bacteriology-bacteriology-fi-a-rop-of-ivksif-cposz-sscrion-fig-38-murrills-gas-pressure-regulator-u-tube-containing-water-the-cylinder-may-be-floated-in-water-but-inasmuch-as-this-is-liable-to-evaporate-it-is-better-to-use-liquid-paraffin-the-apparatus-is-provided-with-two-outflow-tubes-which-are-to-be-connected-with-thermo-regulators-it-is-of-convenient-size-being-only-6-1-journ-of-applied-microscopy-1-p-92-1898-centralblatt-ffir-bakteriologie-23-p-10561898-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-image216420922.htmlRMPG2R2J–. Laboratory work in bacteriology. Bacteriology. fi^ A^- rOP OF /IVKSIf CPOSZ-SSCrioN. Fig. 38. Murrill's gas-pressure regulator. U tube containing water. The cylinder may be floated in water, but, inasmuch as this is liable to evaporate, it is better to use liquid paraffin. The apparatus is provided with two outflow tubes, which are to be connected with thermo-regulators. It is of convenient size, being only 6 1 Journ. of Applied Microscopy 1, p. 92, 1898. Centralblatt ffir Bakteriologie 23, p. 1056,1898.. Please note that these images are extracted from scanned page images that may have been
. Applied bacteriology for nurses. Fig. 11.—Incubator (Eyre). lodged on the wire. The loop cools in a moment, theculture is transferred, the tubes are again closed withthe cotton plugs, and the wire is at once passed throughthe flame before being laid down. Thus all danger ofspreading the germs about is avoided. 30 APPLIED BACTERIOLOGY FOR NURSES The freshly planted culture is now placed in theincubator. This is a kind of oven whose walls are filled. Fig. 12.—Streptococcus pyogenes, culture on agar. Slightly en-larged (Williams). with water, and whose interior is kept at a uniform tem-perature Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/applied-bacteriology-for-nurses-fig-11incubator-eyre-lodged-on-the-wire-the-loop-cools-in-a-moment-theculture-is-transferred-the-tubes-are-again-closed-withthe-cotton-plugs-and-the-wire-is-at-once-passed-throughthe-flame-before-being-laid-down-thus-all-danger-ofspreading-the-germs-about-is-avoided-30-applied-bacteriology-for-nurses-the-freshly-planted-culture-is-now-placed-in-theincubator-this-is-a-kind-of-oven-whose-walls-are-filled-fig-12streptococcus-pyogenes-culture-on-agar-slightly-en-larged-williams-with-water-and-whose-interior-is-kept-at-a-uniform-tem-perature-image336682808.htmlRM2AFN6A0–. Applied bacteriology for nurses. Fig. 11.—Incubator (Eyre). lodged on the wire. The loop cools in a moment, theculture is transferred, the tubes are again closed withthe cotton plugs, and the wire is at once passed throughthe flame before being laid down. Thus all danger ofspreading the germs about is avoided. 30 APPLIED BACTERIOLOGY FOR NURSES The freshly planted culture is now placed in theincubator. This is a kind of oven whose walls are filled. Fig. 12.—Streptococcus pyogenes, culture on agar. Slightly en-larged (Williams). with water, and whose interior is kept at a uniform tem-perature
![. Laboratory work in bacteriology. Bacteriology. Fig. 52. Fig. 53- Figs. 51-53. The author's apparatus tor the culture of anaerobic bacteria. Fig. 51. Bottle for tube cultures. Fig. 52. Apparatus for Petri dishes or tubes.—gas or pyrogal- late method. Fig. 53. Apparatus for plates or tubes,—gas, pyrogallate or vacuum method.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Novy, Frederick George, 1864- . Ann Arbor [Mich. ] Stock Photo](https://c8.alamy.com/comp/PG2R01/laboratory-work-in-bacteriology-bacteriology-fig-52-fig-53-figs-51-53-the-authors-apparatus-tor-the-culture-of-anaerobic-bacteria-fig-51-bottle-for-tube-cultures-fig-52-apparatus-for-petri-dishes-or-tubesgas-or-pyrogal-late-method-fig-53-apparatus-for-plates-or-tubesgas-pyrogallate-or-vacuum-method-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-digitally-enhanced-for-readability-coloration-and-appearance-of-these-illustrations-may-not-perfectly-resemble-the-original-work-novy-frederick-george-1864-ann-arbor-mich-PG2R01.jpg ". Laboratory work in bacteriology. Bacteriology. Fig. 52. Fig. 53- Figs. 51-53. The author's apparatus tor the culture of anaerobic bacteria. Fig. 51. Bottle for tube cultures. Fig. 52. Apparatus for Petri dishes or tubes.—gas or pyrogal- late method. Fig. 53. Apparatus for plates or tubes,—gas, pyrogallate or vacuum method.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Novy, Frederick George, 1864- . Ann Arbor [Mich. ] Stock Photo")
. Laboratory work in bacteriology. Bacteriology. Fig. 52. Fig. 53- Figs. 51-53. The author's apparatus tor the culture of anaerobic bacteria. Fig. 51. Bottle for tube cultures. Fig. 52. Apparatus for Petri dishes or tubes.—gas or pyrogal- late method. Fig. 53. Apparatus for plates or tubes,—gas, pyrogallate or vacuum method.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Novy, Frederick George, 1864- . Ann Arbor [Mich. ] Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/laboratory-work-in-bacteriology-bacteriology-fig-52-fig-53-figs-51-53-the-authors-apparatus-tor-the-culture-of-anaerobic-bacteria-fig-51-bottle-for-tube-cultures-fig-52-apparatus-for-petri-dishes-or-tubesgas-or-pyrogal-late-method-fig-53-apparatus-for-plates-or-tubesgas-pyrogallate-or-vacuum-method-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-digitally-enhanced-for-readability-coloration-and-appearance-of-these-illustrations-may-not-perfectly-resemble-the-original-work-novy-frederick-george-1864-ann-arbor-mich-image216420849.htmlRMPG2R01–. Laboratory work in bacteriology. Bacteriology. Fig. 52. Fig. 53- Figs. 51-53. The author's apparatus tor the culture of anaerobic bacteria. Fig. 51. Bottle for tube cultures. Fig. 52. Apparatus for Petri dishes or tubes.—gas or pyrogal- late method. Fig. 53. Apparatus for plates or tubes,—gas, pyrogallate or vacuum method.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Novy, Frederick George, 1864- . Ann Arbor [Mich. ]
Practical dairy bacteriology, prepared for the use of students, dairymen, and all interested in the problems of the relation of milk to public health . ater put the material again in a steam sterilizer,and sterilize for yi hour. t. 24 hours later sterilize again in steam fpr J^ hour. Thematerial,is now finished and may be preserved for future use. /. Agar slants. After the last steaming, while the agar inthe tubes is still hot and liquid, lay 20 of them down on a tablewith their mouths slightly raised so that the agar will form aslanting surface in the tubes. (Fig. 74.) Allow them to .hardenin Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/practical-dairy-bacteriology-prepared-for-the-use-of-students-dairymen-and-all-interested-in-the-problems-of-the-relation-of-milk-to-public-health-ater-put-the-material-again-in-a-steam-sterilizerand-sterilize-for-yi-hour-t-24-hours-later-sterilize-again-in-steam-fpr-j-hour-thematerialis-now-finished-and-may-be-preserved-for-future-use-agar-slants-after-the-last-steaming-while-the-agar-inthe-tubes-is-still-hot-and-liquid-lay-20-of-them-down-on-a-tablewith-their-mouths-slightly-raised-so-that-the-agar-will-form-aslanting-surface-in-the-tubes-fig-74-allow-them-to-hardenin-image339106799.htmlRM2AKKJ53–Practical dairy bacteriology, prepared for the use of students, dairymen, and all interested in the problems of the relation of milk to public health . ater put the material again in a steam sterilizer,and sterilize for yi hour. t. 24 hours later sterilize again in steam fpr J^ hour. Thematerial,is now finished and may be preserved for future use. /. Agar slants. After the last steaming, while the agar inthe tubes is still hot and liquid, lay 20 of them down on a tablewith their mouths slightly raised so that the agar will form aslanting surface in the tubes. (Fig. 74.) Allow them to .hardenin
. Bacteriology and mycology of foods. Food. 10 BACTERIOLOGICAL APPARATUS of this suspension to a second tube of stenle water. This procedure is repeated until seveial concentrations of the sample ar6 secured. Portions of these are placed in sterile Petri dishes or under these condi- tions put into tubes of melted medmm. These are then put into the Petri dishes. Isolation of Pure Cultures. A pure culture may be defined in different ways but in this relation it may be regarded as the descend- ants of a single cell. Each single cell on the Petri dishes poured m the above paragraph will have devel Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriology-and-mycology-of-foods-food-10-bacteriological-apparatus-of-this-suspension-to-a-second-tube-of-stenle-water-this-procedure-is-repeated-until-seveial-concentrations-of-the-sample-ar6-secured-portions-of-these-are-placed-in-sterile-petri-dishes-or-under-these-condi-tions-put-into-tubes-of-melted-medmm-these-are-then-put-into-the-petri-dishes-isolation-of-pure-cultures-a-pure-culture-may-be-defined-in-different-ways-but-in-this-relation-it-may-be-regarded-as-the-descend-ants-of-a-single-cell-each-single-cell-on-the-petri-dishes-poured-m-the-above-paragraph-will-have-devel-image216324216.htmlRMPFXBMT–. Bacteriology and mycology of foods. Food. 10 BACTERIOLOGICAL APPARATUS of this suspension to a second tube of stenle water. This procedure is repeated until seveial concentrations of the sample ar6 secured. Portions of these are placed in sterile Petri dishes or under these condi- tions put into tubes of melted medmm. These are then put into the Petri dishes. Isolation of Pure Cultures. A pure culture may be defined in different ways but in this relation it may be regarded as the descend- ants of a single cell. Each single cell on the Petri dishes poured m the above paragraph will have devel
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . Fig. 17.—Wire cage. Fig. 18.—Cotton-plugged test-tubes. or a good quality of non-absorbent cotton), the cotton beingeasily sterilized and preventing the entrance of germs fromthe air. Tin-foil may be used to cover the cotton, or caps made ofindia-rubber. Test-tubes.—New test-tubes are washed with hydro-chloric acid and water to neutrahze the alkalinity often pres-ent in fresh glass, or in chromic acid cleaning mixture onehour. (Potassium dichromate, 6; water, 30; sulphu Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/essentials-of-bacteriology-being-a-concise-and-systematic-introduction-to-the-study-of-bacteria-and-allied-microorganisms-fig-17wire-cage-fig-18cotton-plugged-test-tubes-or-a-good-quality-of-non-absorbent-cotton-the-cotton-beingeasily-sterilized-and-preventing-the-entrance-of-germs-fromthe-air-tin-foil-may-be-used-to-cover-the-cotton-or-caps-made-ofindia-rubber-test-tubesnew-test-tubes-are-washed-with-hydro-chloric-acid-and-water-to-neutrahze-the-alkalinity-often-pres-ent-in-fresh-glass-or-in-chromic-acid-cleaning-mixture-onehour-potassium-dichromate-6-water-30-sulphu-image342936697.htmlRM2AWX375–Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . Fig. 17.—Wire cage. Fig. 18.—Cotton-plugged test-tubes. or a good quality of non-absorbent cotton), the cotton beingeasily sterilized and preventing the entrance of germs fromthe air. Tin-foil may be used to cover the cotton, or caps made ofindia-rubber. Test-tubes.—New test-tubes are washed with hydro-chloric acid and water to neutrahze the alkalinity often pres-ent in fresh glass, or in chromic acid cleaning mixture onehour. (Potassium dichromate, 6; water, 30; sulphu
. Laboratory work in bacteriology. Bacteriology. COL^ODIUM SACS. 497 be used in making sacs. This is notably true of celloidin solutions. The film of coUodium must possess a certain degree of elasticity, otherwise it cannot be slipped off the tube on which it is deposited. The tubes should be preferably made of yellow glass and should be about 320 mm. long. The end should be perfectly rounded off, like that of a test-tube. These tubes can be obtained having a diameter of 14, 18, 25 or 30 mm. The 18 mm. tube is the most con- venient one for ordinary purposes.. Fig. 70. The rolling of coUodium s Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/laboratory-work-in-bacteriology-bacteriology-colodium-sacs-497-be-used-in-making-sacs-this-is-notably-true-of-celloidin-solutions-the-film-of-couodium-must-possess-a-certain-degree-of-elasticity-otherwise-it-cannot-be-slipped-off-the-tube-on-which-it-is-deposited-the-tubes-should-be-preferably-made-of-yellow-glass-and-should-be-about-320-mm-long-the-end-should-be-perfectly-rounded-off-like-that-of-a-test-tube-these-tubes-can-be-obtained-having-a-diameter-of-14-18-25-or-30-mm-the-18-mm-tube-is-the-most-con-venient-one-for-ordinary-purposes-fig-70-the-rolling-of-couodium-s-image216400214.htmlRMPG1TK2–. Laboratory work in bacteriology. Bacteriology. COL^ODIUM SACS. 497 be used in making sacs. This is notably true of celloidin solutions. The film of coUodium must possess a certain degree of elasticity, otherwise it cannot be slipped off the tube on which it is deposited. The tubes should be preferably made of yellow glass and should be about 320 mm. long. The end should be perfectly rounded off, like that of a test-tube. These tubes can be obtained having a diameter of 14, 18, 25 or 30 mm. The 18 mm. tube is the most con- venient one for ordinary purposes.. Fig. 70. The rolling of coUodium s
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . ol for plugs. Twenty-five or more Petri dishes. Four platinum needles in glass handles. One-half dozen fermentation tubes. One-half dozen tubes for potato culture. One Novy jar. One animal holder. Three wire boxes for holding tubes. Test-tube rack. The materials must include what is needed for makingculture-media: agar, gelatin, peptone, beef-extract, chemic-ally pure salt. And to this there will be added from time to time suchother apparatus and material as occasion de Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/essentials-of-bacteriology-being-a-concise-and-systematic-introduction-to-the-study-of-bacteria-and-allied-microorganisms-ol-for-plugs-twenty-five-or-more-petri-dishes-four-platinum-needles-in-glass-handles-one-half-dozen-fermentation-tubes-one-half-dozen-tubes-for-potato-culture-one-novy-jar-one-animal-holder-three-wire-boxes-for-holding-tubes-test-tube-rack-the-materials-must-include-what-is-needed-for-makingculture-media-agar-gelatin-peptone-beef-extract-chemic-ally-pure-salt-and-to-this-there-will-be-added-from-time-to-time-suchother-apparatus-and-material-as-occasion-de-image342930985.htmlRM2AWWRY5–Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . ol for plugs. Twenty-five or more Petri dishes. Four platinum needles in glass handles. One-half dozen fermentation tubes. One-half dozen tubes for potato culture. One Novy jar. One animal holder. Three wire boxes for holding tubes. Test-tube rack. The materials must include what is needed for makingculture-media: agar, gelatin, peptone, beef-extract, chemic-ally pure salt. And to this there will be added from time to time suchother apparatus and material as occasion de
. Bacteriology and mycology of foods. Food. BACTERIOLOGICAL APPARATUS of rubber tubing. This ^Uush bulb is filled with platinized asbestos. The cultures are placed in a glass tumbler which is put into the jar. Fig. 21 taken from Smillie's original pubhcation will give the details of the asbestos bulb. Exclusion of Oxygen Seattle's Method. Ordinary test tubes containing plain or car- bohydrate broths are used. Melted sterihzed vaseline is poured into the tube until a plug about | in. long is formed. The broth and vase- line are then boiled for twenty to thirty minutes in order to expel any diss Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriology-and-mycology-of-foods-food-bacteriological-apparatus-of-rubber-tubing-this-uush-bulb-is-filled-with-platinized-asbestos-the-cultures-are-placed-in-a-glass-tumbler-which-is-put-into-the-jar-fig-21-taken-from-smillies-original-pubhcation-will-give-the-details-of-the-asbestos-bulb-exclusion-of-oxygen-seattles-method-ordinary-test-tubes-containing-plain-or-car-bohydrate-broths-are-used-melted-sterihzed-vaseline-is-poured-into-the-tube-until-a-plug-about-in-long-is-formed-the-broth-and-vase-line-are-then-boiled-for-twenty-to-thirty-minutes-in-order-to-expel-any-diss-image216324171.htmlRMPFXBK7–. Bacteriology and mycology of foods. Food. BACTERIOLOGICAL APPARATUS of rubber tubing. This ^Uush bulb is filled with platinized asbestos. The cultures are placed in a glass tumbler which is put into the jar. Fig. 21 taken from Smillie's original pubhcation will give the details of the asbestos bulb. Exclusion of Oxygen Seattle's Method. Ordinary test tubes containing plain or car- bohydrate broths are used. Melted sterihzed vaseline is poured into the tube until a plug about | in. long is formed. The broth and vase- line are then boiled for twenty to thirty minutes in order to expel any diss
![. Applied bacteriology for nurses. Fig. 8.—Media in tubes: a, Broth; 6, agar slant; c, potato (Hiss and Zinsser). mixed with a lot of other bacteria. In other words, itis necessary to obtain a pure culture. We shall assume 27 28 APPLIED BACTERIOLOGY FOR NURSES that we have such a culture; let us now consider themethods by which we continue to grow the same.Our first step is to plant the bacteria either in a fluidculture-medium, such as beef-broth, milk, etc., or on the Fig. 9.—Platinum wires for bacteriologic use. surface of a solid medium, such as gelatin, agar, solidi-fied ])lood-serum, pota Stock Photo](https://c8.alamy.com/comp/2AFN6NH/applied-bacteriology-for-nurses-fig-8media-in-tubes-a-broth-6-agar-slant-c-potato-hiss-and-zinsser-mixed-with-a-lot-of-other-bacteria-in-other-words-itis-necessary-to-obtain-a-pure-culture-we-shall-assume-27-28-applied-bacteriology-for-nurses-that-we-have-such-a-culture-let-us-now-consider-themethods-by-which-we-continue-to-grow-the-sameour-first-step-is-to-plant-the-bacteria-either-in-a-fluidculture-medium-such-as-beef-broth-milk-etc-or-on-the-fig-9platinum-wires-for-bacteriologic-use-surface-of-a-solid-medium-such-as-gelatin-agar-solidi-fied-lood-serum-pota-2AFN6NH.jpg ". Applied bacteriology for nurses. Fig. 8.—Media in tubes: a, Broth; 6, agar slant; c, potato (Hiss and Zinsser). mixed with a lot of other bacteria. In other words, itis necessary to obtain a pure culture. We shall assume 27 28 APPLIED BACTERIOLOGY FOR NURSES that we have such a culture; let us now consider themethods by which we continue to grow the same.Our first step is to plant the bacteria either in a fluidculture-medium, such as beef-broth, milk, etc., or on the Fig. 9.—Platinum wires for bacteriologic use. surface of a solid medium, such as gelatin, agar, solidi-fied ])lood-serum, pota Stock Photo")
. Applied bacteriology for nurses. Fig. 8.—Media in tubes: a, Broth; 6, agar slant; c, potato (Hiss and Zinsser). mixed with a lot of other bacteria. In other words, itis necessary to obtain a pure culture. We shall assume 27 28 APPLIED BACTERIOLOGY FOR NURSES that we have such a culture; let us now consider themethods by which we continue to grow the same.Our first step is to plant the bacteria either in a fluidculture-medium, such as beef-broth, milk, etc., or on the Fig. 9.—Platinum wires for bacteriologic use. surface of a solid medium, such as gelatin, agar, solidi-fied ])lood-serum, pota Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/applied-bacteriology-for-nurses-fig-8media-in-tubes-a-broth-6-agar-slant-c-potato-hiss-and-zinsser-mixed-with-a-lot-of-other-bacteria-in-other-words-itis-necessary-to-obtain-a-pure-culture-we-shall-assume-27-28-applied-bacteriology-for-nurses-that-we-have-such-a-culture-let-us-now-consider-themethods-by-which-we-continue-to-grow-the-sameour-first-step-is-to-plant-the-bacteria-either-in-a-fluidculture-medium-such-as-beef-broth-milk-etc-or-on-the-fig-9platinum-wires-for-bacteriologic-use-surface-of-a-solid-medium-such-as-gelatin-agar-solidi-fied-lood-serum-pota-image336683133.htmlRM2AFN6NH–. Applied bacteriology for nurses. Fig. 8.—Media in tubes: a, Broth; 6, agar slant; c, potato (Hiss and Zinsser). mixed with a lot of other bacteria. In other words, itis necessary to obtain a pure culture. We shall assume 27 28 APPLIED BACTERIOLOGY FOR NURSES that we have such a culture; let us now consider themethods by which we continue to grow the same.Our first step is to plant the bacteria either in a fluidculture-medium, such as beef-broth, milk, etc., or on the Fig. 9.—Platinum wires for bacteriologic use. surface of a solid medium, such as gelatin, agar, solidi-fied ])lood-serum, pota
. Laboratory work in bacteriology. Bacteriology. Fig. 26. The author's steam sterilizer, a—An ordinary Hofmann's iron water-bath, ^18 cm. in diameter; d—Copper sterilizer; c—Support blocks. The copper pail is filled with the gelatin tubes, and is placed on the water-bath, in which the water should be actively boiling. In from five to seven minutes steam will issue rapidly, from the tube in the cover, showing- that the temperature in the interior has reached 100°. The steam- ing is then continued for an additional 15 minutes. This process is now repeated, on each of the following two days. By m Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/laboratory-work-in-bacteriology-bacteriology-fig-26-the-authors-steam-sterilizer-aan-ordinary-hofmanns-iron-water-bath-18-cm-in-diameter-dcopper-sterilizer-csupport-blocks-the-copper-pail-is-filled-with-the-gelatin-tubes-and-is-placed-on-the-water-bath-in-which-the-water-should-be-actively-boiling-in-from-five-to-seven-minutes-steam-will-issue-rapidly-from-the-tube-in-the-cover-showing-that-the-temperature-in-the-interior-has-reached-100-the-steam-ing-is-then-continued-for-an-additional-15-minutes-this-process-is-now-repeated-on-each-of-the-following-two-days-by-m-image216420995.htmlRMPG2R57–. Laboratory work in bacteriology. Bacteriology. Fig. 26. The author's steam sterilizer, a—An ordinary Hofmann's iron water-bath, ^18 cm. in diameter; d—Copper sterilizer; c—Support blocks. The copper pail is filled with the gelatin tubes, and is placed on the water-bath, in which the water should be actively boiling. In from five to seven minutes steam will issue rapidly, from the tube in the cover, showing- that the temperature in the interior has reached 100°. The steam- ing is then continued for an additional 15 minutes. This process is now repeated, on each of the following two days. By m
Practical dairy bacteriology, prepared for the use of students, dairymen, and all interested in the problems of the relation of milk to public health . half hour steaming on three successive days. *No. 27. Fermentation Tubes. To 200 c.c. of bouillon pre-pared as in (4) add 2 grams of lactose (milk sugar). To a sec-ond 200 c.c. add the same amount of dextrose (grape sugar), andto a third lot add saccharose (cane sugar). Dissolve by heating—filter through filter paper and pour into fermentation tubes(Fig. 20), completely filling the closed arm and about one-half the bulb. Sterilize as usual. If Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/practical-dairy-bacteriology-prepared-for-the-use-of-students-dairymen-and-all-interested-in-the-problems-of-the-relation-of-milk-to-public-health-half-hour-steaming-on-three-successive-days-no-27-fermentation-tubes-to-200-cc-of-bouillon-pre-pared-as-in-4-add-2-grams-of-lactose-milk-sugar-to-a-sec-ond-200-cc-add-the-same-amount-of-dextrose-grape-sugar-andto-a-third-lot-add-saccharose-cane-sugar-dissolve-by-heatingfilter-through-filter-paper-and-pour-into-fermentation-tubesfig-20-completely-filling-the-closed-arm-and-about-one-half-the-bulb-sterilize-as-usual-if-image339105489.htmlRM2AKKGE9–Practical dairy bacteriology, prepared for the use of students, dairymen, and all interested in the problems of the relation of milk to public health . half hour steaming on three successive days. *No. 27. Fermentation Tubes. To 200 c.c. of bouillon pre-pared as in (4) add 2 grams of lactose (milk sugar). To a sec-ond 200 c.c. add the same amount of dextrose (grape sugar), andto a third lot add saccharose (cane sugar). Dissolve by heating—filter through filter paper and pour into fermentation tubes(Fig. 20), completely filling the closed arm and about one-half the bulb. Sterilize as usual. If
![. Bacteria, yeasts and molds in the home. Bacteriology; Yeast fungi; Molds (Fungi). APPENDIX DIRECTIONS FOR LABORATORY EXPERIMENTS ^''"EfilLIZINQ 0H*t<lB6R i Apparatus. The experiments here described are all of a simple character. Many of them can be performed without any special apparatus ; but some would need, in addition to test tubes, flasks, and other simple glassware found in any laboratory, a few pieces, as follows. 1. A steam sterilizer. An ordinary steamer such as used in the kitchen will do. A better form is shown in Fig. 8i. 2. A hot-air sterilizer. The best form is shown in Stock Photo](https://c8.alamy.com/comp/PG2R8T/bacteria-yeasts-and-molds-in-the-home-bacteriology-yeast-fungi-molds-fungi-appendix-directions-for-laboratory-experiments-quotefillizinq-0htltlb6r-i-apparatus-the-experiments-here-described-are-all-of-a-simple-character-many-of-them-can-be-performed-without-any-special-apparatus-but-some-would-need-in-addition-to-test-tubes-flasks-and-other-simple-glassware-found-in-any-laboratory-a-few-pieces-as-follows-1-a-steam-sterilizer-an-ordinary-steamer-such-as-used-in-the-kitchen-will-do-a-better-form-is-shown-in-fig-8i-2-a-hot-air-sterilizer-the-best-form-is-shown-in-PG2R8T.jpg ". Bacteria, yeasts and molds in the home. Bacteriology; Yeast fungi; Molds (Fungi). APPENDIX DIRECTIONS FOR LABORATORY EXPERIMENTS ^''"EfilLIZINQ 0H*t<lB6R i Apparatus. The experiments here described are all of a simple character. Many of them can be performed without any special apparatus ; but some would need, in addition to test tubes, flasks, and other simple glassware found in any laboratory, a few pieces, as follows. 1. A steam sterilizer. An ordinary steamer such as used in the kitchen will do. A better form is shown in Fig. 8i. 2. A hot-air sterilizer. The best form is shown in Stock Photo")
. Bacteria, yeasts and molds in the home. Bacteriology; Yeast fungi; Molds (Fungi). APPENDIX DIRECTIONS FOR LABORATORY EXPERIMENTS ^''"EfilLIZINQ 0H*t<lB6R i Apparatus. The experiments here described are all of a simple character. Many of them can be performed without any special apparatus ; but some would need, in addition to test tubes, flasks, and other simple glassware found in any laboratory, a few pieces, as follows. 1. A steam sterilizer. An ordinary steamer such as used in the kitchen will do. A better form is shown in Fig. 8i. 2. A hot-air sterilizer. The best form is shown in Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteria-yeasts-and-molds-in-the-home-bacteriology-yeast-fungi-molds-fungi-appendix-directions-for-laboratory-experiments-quotefillizinq-0htltlb6r-i-apparatus-the-experiments-here-described-are-all-of-a-simple-character-many-of-them-can-be-performed-without-any-special-apparatus-but-some-would-need-in-addition-to-test-tubes-flasks-and-other-simple-glassware-found-in-any-laboratory-a-few-pieces-as-follows-1-a-steam-sterilizer-an-ordinary-steamer-such-as-used-in-the-kitchen-will-do-a-better-form-is-shown-in-fig-8i-2-a-hot-air-sterilizer-the-best-form-is-shown-in-image216421096.htmlRMPG2R8T–. Bacteria, yeasts and molds in the home. Bacteriology; Yeast fungi; Molds (Fungi). APPENDIX DIRECTIONS FOR LABORATORY EXPERIMENTS ^''"EfilLIZINQ 0H*t<lB6R i Apparatus. The experiments here described are all of a simple character. Many of them can be performed without any special apparatus ; but some would need, in addition to test tubes, flasks, and other simple glassware found in any laboratory, a few pieces, as follows. 1. A steam sterilizer. An ordinary steamer such as used in the kitchen will do. A better form is shown in Fig. 8i. 2. A hot-air sterilizer. The best form is shown in
Journal of bacteriology . ining 1 mil .... 1 50 Dried on paper 1 50 In tablet form, in tubes of 10 tablets 1 50 Abortus Antigen (Bacillus of contag-ious abortion), 1 mil ampuls ... 5 00 Positive Serum (Bacillus of contagiousabortion), 10 mil ampuls 3 00 Glanders Antigen—1 mil(c.c.)ampuls 5 00 Positive Glanders Serum—10 mil(c.c.)ampuls 3 00 All orders for laboratory reagents and tests should be sent direct to Philadelphia,as stock is not carried by our branch houses or jobbers, as it is necessary to distributerecently standardized reagents direct from the laboratory. /8631 H. K. MULFORD COMPANY Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/journal-of-bacteriology-ining-1-mil-1-50-dried-on-paper-1-50-in-tablet-form-in-tubes-of-10-tablets-1-50-abortus-antigen-bacillus-of-contag-ious-abortion-1-mil-ampuls-5-00-positive-serum-bacillus-of-contagiousabortion-10-mil-ampuls-3-00-glanders-antigen1-milccampuls-5-00-positive-glanders-serum10-milccampuls-3-00-all-orders-for-laboratory-reagents-and-tests-should-be-sent-direct-to-philadelphiaas-stock-is-not-carried-by-our-branch-houses-or-jobbers-as-it-is-necessary-to-distributerecently-standardized-reagents-direct-from-the-laboratory-8631-h-k-mulford-company-image338211748.htmlRM2AJ6TF0–Journal of bacteriology . ining 1 mil .... 1 50 Dried on paper 1 50 In tablet form, in tubes of 10 tablets 1 50 Abortus Antigen (Bacillus of contag-ious abortion), 1 mil ampuls ... 5 00 Positive Serum (Bacillus of contagiousabortion), 10 mil ampuls 3 00 Glanders Antigen—1 mil(c.c.)ampuls 5 00 Positive Glanders Serum—10 mil(c.c.)ampuls 3 00 All orders for laboratory reagents and tests should be sent direct to Philadelphia,as stock is not carried by our branch houses or jobbers, as it is necessary to distributerecently standardized reagents direct from the laboratory. /8631 H. K. MULFORD COMPANY
. Laboratory work in bacteriology. Bacteriology. 314 BACTEEIOLOGY. is placed in a position so that only a narrow slit remains open. By means of a pi- pette or other arrangement 25 c.c. of concentrated potash solution (1:4), are -^ added as rapidly as possi- ble. The top is then closed completely and the rubber band and clamps applied as before. Laboratory work. — Liquefy four- glucose agar tubes by heat- ing in the water-bath; then allow to solidify in an upright position. When cool make deep stab cul- tures of the 4 pathogenic anae- robic organisms and then place the tubes at 37°. If the agar Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/laboratory-work-in-bacteriology-bacteriology-314-bacteeiology-is-placed-in-a-position-so-that-only-a-narrow-slit-remains-open-by-means-of-a-pi-pette-or-other-arrangement-25-cc-of-concentrated-potash-solution-14-are-added-as-rapidly-as-possi-ble-the-top-is-then-closed-completely-and-the-rubber-band-and-clamps-applied-as-before-laboratory-work-liquefy-four-glucose-agar-tubes-by-heat-ing-in-the-water-bath-then-allow-to-solidify-in-an-upright-position-when-cool-make-deep-stab-cul-tures-of-the-4-pathogenic-anae-robic-organisms-and-then-place-the-tubes-at-37-if-the-agar-image216420855.htmlRMPG2R07–. Laboratory work in bacteriology. Bacteriology. 314 BACTEEIOLOGY. is placed in a position so that only a narrow slit remains open. By means of a pi- pette or other arrangement 25 c.c. of concentrated potash solution (1:4), are -^ added as rapidly as possi- ble. The top is then closed completely and the rubber band and clamps applied as before. Laboratory work. — Liquefy four- glucose agar tubes by heat- ing in the water-bath; then allow to solidify in an upright position. When cool make deep stab cul- tures of the 4 pathogenic anae- robic organisms and then place the tubes at 37°. If the agar
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. 10 LABORATORY GUIDE IN BACTERIOLOGY at a temperature of 150° C. for about 30 minutes, oruntil the plugs are -slightly browned. The tubes arenot necessarily sterile, but the plugs have become setso as to fit the mouth of the tube, and maybe removedand replaced readily. SECTION 4 METHODS OF STERILIZATION Sterilization is the process of removing all livingorganisms. This may be accomplished by heat, by. Fig. 3Berkefeld Filtera. Berkefeld filter d. Intercepting flask 6. Filtered liquid e. Connection with as Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-10-laboratory-guide-in-bacteriology-at-a-temperature-of-150-c-for-about-30-minutes-oruntil-the-plugs-are-slightly-browned-the-tubes-arenot-necessarily-sterile-but-the-plugs-have-become-setso-as-to-fit-the-mouth-of-the-tube-and-maybe-removedand-replaced-readily-section-4-methods-of-sterilization-sterilization-is-the-process-of-removing-all-livingorganisms-this-may-be-accomplished-by-heat-by-fig-3berkefeld-filtera-berkefeld-filter-d-intercepting-flask-6-filtered-liquid-e-connection-with-as-image370323385.htmlRM2CEDK75–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. 10 LABORATORY GUIDE IN BACTERIOLOGY at a temperature of 150° C. for about 30 minutes, oruntil the plugs are -slightly browned. The tubes arenot necessarily sterile, but the plugs have become setso as to fit the mouth of the tube, and maybe removedand replaced readily. SECTION 4 METHODS OF STERILIZATION Sterilization is the process of removing all livingorganisms. This may be accomplished by heat, by. Fig. 3Berkefeld Filtera. Berkefeld filter d. Intercepting flask 6. Filtered liquid e. Connection with as
. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. Fig. 221. Fig. 222. Fig. 221.—Method of holding test-tubes in transfer of fungi from one test-tube to another. {After Lohnis.) Fig. 222.—Cylindric form of wire basket for holding test-tubes during steriliza- tion and other operations. (After Schneider. Pharmaceutical Bacteriology, p. 37.) A, B and C are thoroughly shaken and each is transferred to Petri dishes marked A, B and C. If water is used to dilute, or bouillon, it must be mixed with the material poured into the Petri dishes. The Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-mycology-and-plant-pathology-plant-diseases-fungi-in-agriculture-plant-diseases-fungi-fig-221-fig-222-fig-221method-of-holding-test-tubes-in-transfer-of-fungi-from-one-test-tube-to-another-after-lohnis-fig-222cylindric-form-of-wire-basket-for-holding-test-tubes-during-steriliza-tion-and-other-operations-after-schneider-pharmaceutical-bacteriology-p-37-a-b-and-c-are-thoroughly-shaken-and-each-is-transferred-to-petri-dishes-marked-a-b-and-c-if-water-is-used-to-dilute-or-bouillon-it-must-be-mixed-with-the-material-poured-into-the-petri-dishes-the-image216457483.htmlRMPG4DMB–. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. Fig. 221. Fig. 222. Fig. 221.—Method of holding test-tubes in transfer of fungi from one test-tube to another. {After Lohnis.) Fig. 222.—Cylindric form of wire basket for holding test-tubes during steriliza- tion and other operations. (After Schneider. Pharmaceutical Bacteriology, p. 37.) A, B and C are thoroughly shaken and each is transferred to Petri dishes marked A, B and C. If water is used to dilute, or bouillon, it must be mixed with the material poured into the Petri dishes. The
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. BACTERIOLOGICAL TECHNIC 17 for one hour. This process has to be repeated for fiveor six successive days, before sterilization is complete.If the autoclave is to be used, the tubes are placedin the autoclave in an inclined position.Good results are obtained by this method:. Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-bacteriological-technic-17-for-one-hour-this-process-has-to-be-repeated-for-fiveor-six-successive-days-before-sterilization-is-completeif-the-autoclave-is-to-be-used-the-tubes-are-placedin-the-autoclave-in-an-inclined-positiongood-results-are-obtained-by-this-method-image370323062.htmlRM2CEDJRJ–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. BACTERIOLOGICAL TECHNIC 17 for one hour. This process has to be repeated for fiveor six successive days, before sterilization is complete.If the autoclave is to be used, the tubes are placedin the autoclave in an inclined position.Good results are obtained by this method:.
. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. 636 LABORATORY EXERCISES III. PHYSICAL AND BIOCHEMIC FEATURES ' I » I ' CO , « . O I. Fermentation Tubes Containing Peptone S a Water or Sugar-fkeb Bouillon, and '-go ; Q to I 's S jo |S I Gas production in per cent. (Fig. 226). (coj i , ^ i 1 Growth in closed arm ' 1 1 ' Amount of acid produced I day 1 1 Amount of acid produced, 2 days 1 1 1 Amount of add produced, 3 days 'â. Fig. 226.âGraduated fennentation tubes for gas determinations. {Schneider, Pharmaceutical Bacteriology, p. 60 Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-mycology-and-plant-pathology-plant-diseases-fungi-in-agriculture-plant-diseases-fungi-636-laboratory-exercises-iii-physical-and-biochemic-features-i-i-co-o-i-fermentation-tubes-containing-peptone-s-a-water-or-sugar-fkeb-bouillon-and-go-q-to-i-s-s-jo-s-i-gas-production-in-per-cent-fig-226-coj-i-i-1-growth-in-closed-arm-1-1-amount-of-acid-produced-i-day-1-1-amount-of-acid-produced-2-days-1-1-1-amount-of-add-produced-3-days-fig-226graduated-fennentation-tubes-for-gas-determinations-schneider-pharmaceutical-bacteriology-p-60-image216457463.htmlRMPG4DKK–. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. 636 LABORATORY EXERCISES III. PHYSICAL AND BIOCHEMIC FEATURES ' I » I ' CO , « . O I. Fermentation Tubes Containing Peptone S a Water or Sugar-fkeb Bouillon, and '-go ; Q to I 's S jo |S I Gas production in per cent. (Fig. 226). (coj i , ^ i 1 Growth in closed arm ' 1 1 ' Amount of acid produced I day 1 1 Amount of acid produced, 2 days 1 1 1 Amount of add produced, 3 days 'â. Fig. 226.âGraduated fennentation tubes for gas determinations. {Schneider, Pharmaceutical Bacteriology, p. 60
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. other glassware should be treated in the samemanner, excepting fermentation tubes, which shouldnot be heated in the hot-air oven, as this would belikely to cause breakage. The tubes should be plugged with cotton. Non-absorbent cotton is suitable for this purpose. Thecotton plug allows free communication with the air,admitting oxygen, which is necessary for the growthof many bacteria; at the same time the admitted air isfiltered germ-free and contamination of cultures isavoided. Various methods for plugg Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-other-glassware-should-be-treated-in-the-samemanner-excepting-fermentation-tubes-which-shouldnot-be-heated-in-the-hot-air-oven-as-this-would-belikely-to-cause-breakage-the-tubes-should-be-plugged-with-cotton-non-absorbent-cotton-is-suitable-for-this-purpose-thecotton-plug-allows-free-communication-with-the-airadmitting-oxygen-which-is-necessary-for-the-growthof-many-bacteria-at-the-same-time-the-admitted-air-isfiltered-germ-free-and-contamination-of-cultures-isavoided-various-methods-for-plugg-image370323539.htmlRM2CEDKCK–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. other glassware should be treated in the samemanner, excepting fermentation tubes, which shouldnot be heated in the hot-air oven, as this would belikely to cause breakage. The tubes should be plugged with cotton. Non-absorbent cotton is suitable for this purpose. Thecotton plug allows free communication with the air,admitting oxygen, which is necessary for the growthof many bacteria; at the same time the admitted air isfiltered germ-free and contamination of cultures isavoided. Various methods for plugg
. A manual of clinical diagnosis by means of microscopical and chemical methods, for students, hospital physicians, and practitioners . To examine the blood, a rubber tube, provided with a mouth-piece (Fig. 26), is slipped over the end of one of the glass tubes,and the tube filled completely by suction from a drop of bloodobtained from the finger or the ear. The blunt point of the tube BACTERIOLOGY AND PARASITOLOGY OF THE BLOOD. 113 is covered quickly with the finger and the tube fixed in the frame.This is rotated at a speed of 10,000 revolutions for two or threeminutes, when the volume of the Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-manual-of-clinical-diagnosis-by-means-of-microscopical-and-chemical-methods-for-students-hospital-physicians-and-practitioners-to-examine-the-blood-a-rubber-tube-provided-with-a-mouth-piece-fig-26-is-slipped-over-the-end-of-one-of-the-glass-tubesand-the-tube-filled-completely-by-suction-from-a-drop-of-bloodobtained-from-the-finger-or-the-ear-the-blunt-point-of-the-tube-bacteriology-and-parasitology-of-the-blood-113-is-covered-quickly-with-the-finger-and-the-tube-fixed-in-the-framethis-is-rotated-at-a-speed-of-10000-revolutions-for-two-or-threeminutes-when-the-volume-of-the-image372540486.htmlRM2CJ2K5A–. A manual of clinical diagnosis by means of microscopical and chemical methods, for students, hospital physicians, and practitioners . To examine the blood, a rubber tube, provided with a mouth-piece (Fig. 26), is slipped over the end of one of the glass tubes,and the tube filled completely by suction from a drop of bloodobtained from the finger or the ear. The blunt point of the tube BACTERIOLOGY AND PARASITOLOGY OF THE BLOOD. 113 is covered quickly with the finger and the tube fixed in the frame.This is rotated at a speed of 10,000 revolutions for two or threeminutes, when the volume of the
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . n— 1. Singe the cotton plug of a tube containing themedium. It is imperative always to singe the cottonplugs of tubes before opening them, whether these tubescontain a culture or a sterile medium. The organismsfrom the air are constantly falling on the cotton, andbacteria may also be deposited on the cotton by handlingit with the fingers. If these organisms were not killed bythe process of singeing, they would drop on the mediumafter removal of the stopper, and thus ruin a pure cuhure. 2. Hold the tube Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-n-1-singe-the-cotton-plug-of-a-tube-containing-themedium-it-is-imperative-always-to-singe-the-cottonplugs-of-tubes-before-opening-them-whether-these-tubescontain-a-culture-or-a-sterile-medium-the-organismsfrom-the-air-are-constantly-falling-on-the-cotton-andbacteria-may-also-be-deposited-on-the-cotton-by-handlingit-with-the-fingers-if-these-organisms-were-not-killed-bythe-process-of-singeing-they-would-drop-on-the-mediumafter-removal-of-the-stopper-and-thus-ruin-a-pure-cuhure-2-hold-the-tube-image370176215.htmlRM2CE6YF3–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . n— 1. Singe the cotton plug of a tube containing themedium. It is imperative always to singe the cottonplugs of tubes before opening them, whether these tubescontain a culture or a sterile medium. The organismsfrom the air are constantly falling on the cotton, andbacteria may also be deposited on the cotton by handlingit with the fingers. If these organisms were not killed bythe process of singeing, they would drop on the mediumafter removal of the stopper, and thus ruin a pure cuhure. 2. Hold the tube
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. reactions which take place in the culture-media. When the reaction has been sufficiently adjusted, themedium is to be filtered and is then ready to be distributedin test-tubes. The methods of making the normal and twentieth normalsolutions required may be found in standard works on chem-istry. If one has not some knowledge of chemistry, hewould better have the solutions made by a chemist. The filling of the test-tubes with the fluid culture-media described in this section is best effected by me Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-reactions-which-take-place-in-the-culture-media-when-the-reaction-has-been-sufficiently-adjusted-themedium-is-to-be-filtered-and-is-then-ready-to-be-distributedin-test-tubes-the-methods-of-making-the-normal-and-twentieth-normalsolutions-required-may-be-found-in-standard-works-on-chem-istry-if-one-has-not-some-knowledge-of-chemistry-hewould-better-have-the-solutions-made-by-a-chemist-the-filling-of-the-test-tubes-with-the-fluid-culture-media-described-in-this-section-is-best-effected-by-me-image370420418.htmlRM2CEJ30J–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. reactions which take place in the culture-media. When the reaction has been sufficiently adjusted, themedium is to be filtered and is then ready to be distributedin test-tubes. The methods of making the normal and twentieth normalsolutions required may be found in standard works on chem-istry. If one has not some knowledge of chemistry, hewould better have the solutions made by a chemist. The filling of the test-tubes with the fluid culture-media described in this section is best effected by me
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . Fig. 3Kochs Hot-Air Sterilizer 10 LABORATORY GUIDE IN BACTERIOLOGY should enter the hole provided at the bottom of thesterilizer for that purpose. Care should be taken toavoid the possibility of the flame becoming luminous,as this would fill the box and cover the glassware withsoot (Figs. 3 and 4). The plugged culture-tubes are then placed in the. Fig. 4Lautenschlager Hot-Air Sterilizer sterilizer, and a temperature of 150° is maintained forabout 30 minutes, or until the plugs are slightly charred.This Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-fig-3kochs-hot-air-sterilizer-10-laboratory-guide-in-bacteriology-should-enter-the-hole-provided-at-the-bottom-of-thesterilizer-for-that-purpose-care-should-be-taken-toavoid-the-possibility-of-the-flame-becoming-luminousas-this-would-fill-the-box-and-cover-the-glassware-withsoot-figs-3-and-4-the-plugged-culture-tubes-are-then-placed-in-the-fig-4lautenschlager-hot-air-sterilizer-sterilizer-and-a-temperature-of-150-is-maintained-forabout-30-minutes-or-until-the-plugs-are-slightly-charredthis-image370176898.htmlRM2CE70BE–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . Fig. 3Kochs Hot-Air Sterilizer 10 LABORATORY GUIDE IN BACTERIOLOGY should enter the hole provided at the bottom of thesterilizer for that purpose. Care should be taken toavoid the possibility of the flame becoming luminous,as this would fill the box and cover the glassware withsoot (Figs. 3 and 4). The plugged culture-tubes are then placed in the. Fig. 4Lautenschlager Hot-Air Sterilizer sterilizer, and a temperature of 150° is maintained forabout 30 minutes, or until the plugs are slightly charred.This
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. bydirect extension from a tuberculous focus in the lung) oftenshows a large number or even a predominance of poly-nuclear leucocytes, owing to a secondary infection withpyogenic bacteria. Old mechanical effusions are occasionally encounteredwhere little endothelium is seen, and the cells found arealmost exclusively lymphocytes. Method.—The fluid should be drawn with the usualaseptic precautions into sterilized flasks or tubes. If it isclotted, it should be shaken until the clot is thoroughly co Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-bydirect-extension-from-a-tuberculous-focus-in-the-lung-oftenshows-a-large-number-or-even-a-predominance-of-poly-nuclear-leucocytes-owing-to-a-secondary-infection-withpyogenic-bacteria-old-mechanical-effusions-are-occasionally-encounteredwhere-little-endothelium-is-seen-and-the-cells-found-arealmost-exclusively-lymphocytes-methodthe-fluid-should-be-drawn-with-the-usualaseptic-precautions-into-sterilized-flasks-or-tubes-if-it-isclotted-it-should-be-shaken-until-the-clot-is-thoroughly-co-image370400786.htmlRM2CEH5YE–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. bydirect extension from a tuberculous focus in the lung) oftenshows a large number or even a predominance of poly-nuclear leucocytes, owing to a secondary infection withpyogenic bacteria. Old mechanical effusions are occasionally encounteredwhere little endothelium is seen, and the cells found arealmost exclusively lymphocytes. Method.—The fluid should be drawn with the usualaseptic precautions into sterilized flasks or tubes. If it isclotted, it should be shaken until the clot is thoroughly co
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. g position in such away as to give a good view to the operator of the surface ofthe media in each, while the cotton stoppers are removedand held between the fingers of the same hand (Fig. 19).The object of holding the tubes in a slanting position is tooffer less chance of contamination from bacteria gaining en-trance to the culture-medium from the air. The platinum wire, which is manipulated by the righthand, is first sterilized by holding in the Bunsen flame untilit glows, and then cooled by c Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-g-position-in-such-away-as-to-give-a-good-view-to-the-operator-of-the-surface-ofthe-media-in-each-while-the-cotton-stoppers-are-removedand-held-between-the-fingers-of-the-same-hand-fig-19the-object-of-holding-the-tubes-in-a-slanting-position-is-tooffer-less-chance-of-contamination-from-bacteria-gaining-en-trance-to-the-culture-medium-from-the-air-the-platinum-wire-which-is-manipulated-by-the-righthand-is-first-sterilized-by-holding-in-the-bunsen-flame-untilit-glows-and-then-cooled-by-c-image370419699.htmlRM2CEJ22Y–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. g position in such away as to give a good view to the operator of the surface ofthe media in each, while the cotton stoppers are removedand held between the fingers of the same hand (Fig. 19).The object of holding the tubes in a slanting position is tooffer less chance of contamination from bacteria gaining en-trance to the culture-medium from the air. The platinum wire, which is manipulated by the righthand, is first sterilized by holding in the Bunsen flame untilit glows, and then cooled by c
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. (Fig. 29, a). This flask is provided with a rubberstopper (6) with two holes, through which two glasstubes (c with a wide opening and d) lead. Cottonplugs are then inserted at c and d, and the apparatusis sterilized in the autoclave. A large bottle (/), con-taining 5 liters of water, is then provided with a rubberstopper, and also with two glass tubes (g and h); hisconnected with a short piece of rubber hose and apinchcock {i). When the Erlenmeyer flask and con-tents are sterilized, the tube d is connec Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-fig-29-a-this-flask-is-provided-with-a-rubberstopper-6-with-two-holes-through-which-two-glasstubes-c-with-a-wide-opening-and-d-lead-cottonplugs-are-then-inserted-at-c-and-d-and-the-apparatusis-sterilized-in-the-autoclave-a-large-bottle-con-taining-5-liters-of-water-is-then-provided-with-a-rubberstopper-and-also-with-two-glass-tubes-g-and-h-hisconnected-with-a-short-piece-of-rubber-hose-and-apinchcock-i-when-the-erlenmeyer-flask-and-con-tents-are-sterilized-the-tube-d-is-connec-image370322001.htmlRM2CEDHDN–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. (Fig. 29, a). This flask is provided with a rubberstopper (6) with two holes, through which two glasstubes (c with a wide opening and d) lead. Cottonplugs are then inserted at c and d, and the apparatusis sterilized in the autoclave. A large bottle (/), con-taining 5 liters of water, is then provided with a rubberstopper, and also with two glass tubes (g and h); hisconnected with a short piece of rubber hose and apinchcock {i). When the Erlenmeyer flask and con-tents are sterilized, the tube d is connec
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. species is that known as the plate method of Petri. This isa modification of the original complicated method of Koch. The method consists in making dilutions in meltedagar-agar or gelatin tubes, and then pouring the infectedmedium into shallow glass dishes (Fig. 21) previously steril-ized, in which it is allowed to solidify. A few bacteria arethus distributed throughout a thin layer of culture-medium CULTURE METHODS. 219 in the dilutions, and the colonies which develop from themare then more or Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-species-is-that-known-as-the-plate-method-of-petri-this-isa-modification-of-the-original-complicated-method-of-koch-the-method-consists-in-making-dilutions-in-meltedagar-agar-or-gelatin-tubes-and-then-pouring-the-infectedmedium-into-shallow-glass-dishes-fig-21-previously-steril-ized-in-which-it-is-allowed-to-solidify-a-few-bacteria-arethus-distributed-throughout-a-thin-layer-of-culture-medium-culture-methods-219-in-the-dilutions-and-the-colonies-which-develop-from-themare-then-more-or-image370419403.htmlRM2CEJ1MB–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. species is that known as the plate method of Petri. This isa modification of the original complicated method of Koch. The method consists in making dilutions in meltedagar-agar or gelatin tubes, and then pouring the infectedmedium into shallow glass dishes (Fig. 21) previously steril-ized, in which it is allowed to solidify. A few bacteria arethus distributed throughout a thin layer of culture-medium CULTURE METHODS. 219 in the dilutions, and the colonies which develop from themare then more or
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . ugh to allow a culture-tube toslip in easily. A thermometer is passed through a rubbercork with a hole in its center, and inserted into oneof the holes in the water-bath. Enough water is thenpoured into the apparatus to fill it about two-thirds, andthe thermometer is lowered until the mercury bulb is 37 38 LABORATORY GUIDE IN BACTERIOLOGY completely immersed in the water. The culture-tubesare then sUpped in, and the water is heated to loo?. 3. Singe the cotton stopper of the liquefied agar-tubes in the Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-ugh-to-allow-a-culture-tube-toslip-in-easily-a-thermometer-is-passed-through-a-rubbercork-with-a-hole-in-its-center-and-inserted-into-oneof-the-holes-in-the-water-bath-enough-water-is-thenpoured-into-the-apparatus-to-fill-it-about-two-thirds-andthe-thermometer-is-lowered-until-the-mercury-bulb-is-37-38-laboratory-guide-in-bacteriology-completely-immersed-in-the-water-the-culture-tubesare-then-supped-in-and-the-water-is-heated-to-loo-3-singe-the-cotton-stopper-of-the-liquefied-agar-tubes-in-the-image370176348.htmlRM2CE6YKT–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . ugh to allow a culture-tube toslip in easily. A thermometer is passed through a rubbercork with a hole in its center, and inserted into oneof the holes in the water-bath. Enough water is thenpoured into the apparatus to fill it about two-thirds, andthe thermometer is lowered until the mercury bulb is 37 38 LABORATORY GUIDE IN BACTERIOLOGY completely immersed in the water. The culture-tubesare then sUpped in, and the water is heated to loo?. 3. Singe the cotton stopper of the liquefied agar-tubes in the
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . method is employed, the filter must alwaysfirst be soaked in hot water, or, better, kept in steamuntil used. PREPARATION OF CULTURE MEDIA 19 The filtered liquid is collected in a smaller funnel(Fig- S> 0- A wire basket should be supported so as tobe inclined to an angle of about 45°, so as to allow theproper arrangement of the tubes. Remove the plug of a cuhure-tube and quickly passthe latter up the full length of the pipette (Fig. 5, /), whichshould be no less than 3 inches long. Open the pinch-coc Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-method-is-employed-the-filter-must-alwaysfirst-be-soaked-in-hot-water-or-better-kept-in-steamuntil-used-preparation-of-culture-media-19-the-filtered-liquid-is-collected-in-a-smaller-funnelfig-sgt-0-a-wire-basket-should-be-supported-so-as-tobe-inclined-to-an-angle-of-about-45-so-as-to-allow-theproper-arrangement-of-the-tubes-remove-the-plug-of-a-cuhure-tube-and-quickly-passthe-latter-up-the-full-length-of-the-pipette-fig-5-whichshould-be-no-less-than-3-inches-long-open-the-pinch-coc-image370176611.htmlRM2CE7017–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . method is employed, the filter must alwaysfirst be soaked in hot water, or, better, kept in steamuntil used. PREPARATION OF CULTURE MEDIA 19 The filtered liquid is collected in a smaller funnel(Fig- S> 0- A wire basket should be supported so as tobe inclined to an angle of about 45°, so as to allow theproper arrangement of the tubes. Remove the plug of a cuhure-tube and quickly passthe latter up the full length of the pipette (Fig. 5, /), whichshould be no less than 3 inches long. Open the pinch-coc
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. lcium carbonate. With the bouillon cultures so obtainedagglutination tests with immune serum should be carriedout in dilutions of 1 : 100, 1 : 200, 1 : 400. Mixtures ofserum dilutions and bouillon culture of 0.5 c.c. each areplaced in small tubes and incubated at 550 C. for twenty-four hours. Bacillus of Influenza.—Morphology.—Very small ba-cilli, with rounded ends and of variable length, sometimesgrowing into long forms, more or less bent or curved (see Fig- 55). Stains more deeply at the ends Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-lcium-carbonate-with-the-bouillon-cultures-so-obtainedagglutination-tests-with-immune-serum-should-be-carriedout-in-dilutions-of-1-100-1-200-1-400-mixtures-ofserum-dilutions-and-bouillon-culture-of-05-cc-each-areplaced-in-small-tubes-and-incubated-at-550-c-for-twenty-four-hours-bacillus-of-influenzamorphologyvery-small-ba-cilli-with-rounded-ends-and-of-variable-length-sometimesgrowing-into-long-forms-more-or-less-bent-or-curved-see-fig-55-stains-more-deeply-at-the-ends-image370413317.htmlRM2CEHNY1–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. lcium carbonate. With the bouillon cultures so obtainedagglutination tests with immune serum should be carriedout in dilutions of 1 : 100, 1 : 200, 1 : 400. Mixtures ofserum dilutions and bouillon culture of 0.5 c.c. each areplaced in small tubes and incubated at 550 C. for twenty-four hours. Bacillus of Influenza.—Morphology.—Very small ba-cilli, with rounded ends and of variable length, sometimesgrowing into long forms, more or less bent or curved (see Fig- 55). Stains more deeply at the ends
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. rogen gas,which can be ascertained by holding a culture tubeover the opening (/) and then over a burning matchor gas flame. As long as a detonation takes place thehydrogen is mixed with atmospheric oxygen. Whenthe hydrogen in the Novy jar is pure, close it off byturning the stoppers (g) and (6), and place the jar inthe incubator. The whole process occupies 10 or 15minutes. 5. A simple and effective method of anaerobic culti-vation is as follows: Culture tubes, after inoculation,are placed in a desiccati Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-rogen-gaswhich-can-be-ascertained-by-holding-a-culture-tubeover-the-opening-and-then-over-a-burning-matchor-gas-flame-as-long-as-a-detonation-takes-place-thehydrogen-is-mixed-with-atmospheric-oxygen-whenthe-hydrogen-in-the-novy-jar-is-pure-close-it-off-byturning-the-stoppers-g-and-6-and-place-the-jar-inthe-incubator-the-whole-process-occupies-10-or-15minutes-5-a-simple-and-effective-method-of-anaerobic-culti-vation-is-as-follows-culture-tubes-after-inoculationare-placed-in-a-desiccati-image370321800.htmlRM2CEDH6G–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. rogen gas,which can be ascertained by holding a culture tubeover the opening (/) and then over a burning matchor gas flame. As long as a detonation takes place thehydrogen is mixed with atmospheric oxygen. Whenthe hydrogen in the Novy jar is pure, close it off byturning the stoppers (g) and (6), and place the jar inthe incubator. The whole process occupies 10 or 15minutes. 5. A simple and effective method of anaerobic culti-vation is as follows: Culture tubes, after inoculation,are placed in a desiccati
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. n the tubes,it will probably be very difficult or impossible to isolate thespecific micro-organism. If there are a few or no contami-nating colonies, then the colonies of the specific micro-organ- 24 37° PA THOL O GICA L TE CffNIQ UE. ism should be expected to develop in the course of two orthree days to a week. If a good number of living filamentsof the micro-organism have been distributed throughout theagar, the specific colonies that develop will be very numer-ous in the depths of the agar, Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-n-the-tubesit-will-probably-be-very-difficult-or-impossible-to-isolate-thespecific-micro-organism-if-there-are-a-few-or-no-contami-nating-colonies-then-the-colonies-of-the-specific-micro-organ-24-37-pa-thol-o-gica-l-te-cffniq-ue-ism-should-be-expected-to-develop-in-the-course-of-two-orthree-days-to-a-week-if-a-good-number-of-living-filamentsof-the-micro-organism-have-been-distributed-throughout-theagar-the-specific-colonies-that-develop-will-be-very-numer-ous-in-the-depths-of-the-agar-image370406836.htmlRM2CEHDKG–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. n the tubes,it will probably be very difficult or impossible to isolate thespecific micro-organism. If there are a few or no contami-nating colonies, then the colonies of the specific micro-organ- 24 37° PA THOL O GICA L TE CffNIQ UE. ism should be expected to develop in the course of two orthree days to a week. If a good number of living filamentsof the micro-organism have been distributed throughout theagar, the specific colonies that develop will be very numer-ous in the depths of the agar,
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. e should be turned carefully, so that the gasmay escape slowly and evenly. Tissues fixed by alcohol orany other reagent, except formaldehyde, must be washed inrunning water for some hours before they can be frozen. Even for tissue fixed in formaldehyd washing in water forten to thirty minutes is advisable, as better sections can beobtained. It is now possible to obtain from stores carrying automo-bile supplies small tubes of compressed carbon dioxid suf-ficient for one or two freezings. They wi Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-e-should-be-turned-carefully-so-that-the-gasmay-escape-slowly-and-evenly-tissues-fixed-by-alcohol-orany-other-reagent-except-formaldehyde-must-be-washed-inrunning-water-for-some-hours-before-they-can-be-frozen-even-for-tissue-fixed-in-formaldehyd-washing-in-water-forten-to-thirty-minutes-is-advisable-as-better-sections-can-beobtained-it-is-now-possible-to-obtain-from-stores-carrying-automo-bile-supplies-small-tubes-of-compressed-carbon-dioxid-suf-ficient-for-one-or-two-freezings-they-wi-image370423388.htmlRM2CEJ6PM–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. e should be turned carefully, so that the gasmay escape slowly and evenly. Tissues fixed by alcohol orany other reagent, except formaldehyde, must be washed inrunning water for some hours before they can be frozen. Even for tissue fixed in formaldehyd washing in water forten to thirty minutes is advisable, as better sections can beobtained. It is now possible to obtain from stores carrying automo-bile supplies small tubes of compressed carbon dioxid suf-ficient for one or two freezings. They wi
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. tion of a second sterileblood-serum tube, next touched to the infected surface of the FIG. 19—Method of holding tubes dur-ing inoculation. 218 PATHOLOGICAL TECHNIQUE. first tube, and the wire thus infected gently and thoroughlyrubbed over the surface of the second. In a similar mannera third tube is then infected from the second, and then all thetubes placed in the incubator for eighteen to twenty-four hours.It is evident that comparatively few bacteria will be sown onthe medium of the second t Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-tion-of-a-second-sterileblood-serum-tube-next-touched-to-the-infected-surface-of-the-fig-19method-of-holding-tubes-dur-ing-inoculation-218-pathological-technique-first-tube-and-the-wire-thus-infected-gently-and-thoroughlyrubbed-over-the-surface-of-the-second-in-a-similar-mannera-third-tube-is-then-infected-from-the-second-and-then-all-thetubes-placed-in-the-incubator-for-eighteen-to-twenty-four-hoursit-is-evident-that-comparatively-few-bacteria-will-be-sown-onthe-medium-of-the-second-t-image370419621.htmlRM2CEJ205–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. tion of a second sterileblood-serum tube, next touched to the infected surface of the FIG. 19—Method of holding tubes dur-ing inoculation. 218 PATHOLOGICAL TECHNIQUE. first tube, and the wire thus infected gently and thoroughlyrubbed over the surface of the second. In a similar mannera third tube is then infected from the second, and then all thetubes placed in the incubator for eighteen to twenty-four hours.It is evident that comparatively few bacteria will be sown onthe medium of the second t
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . Fig. 17Ordinary Style of Potato-Tubeu.. Potato 4. Cotton 2. Add 7-10 per cent, of a solution of litmus (tinctureof litmus). 3. Distribute about 7 c.c. into each of twenty-five tubes. 4. Sterilize in autoclav for 5 minutes at 120°, or inArnold for 3 consecutive days. Note.—The prepared culture media should be carefullystored in a dark, cool place. If they are to be kept for a consid- PREPARATION OF CULTURE MEDIA 29 erable length of time, they should be sealed either with paraffinor with a rubber cap. Th Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-fig-17ordinary-style-of-potato-tubeu-potato-4-cotton-2-add-7-10-per-cent-of-a-solution-of-litmus-tinctureof-litmus-3-distribute-about-7-cc-into-each-of-twenty-five-tubes-4-sterilize-in-autoclav-for-5-minutes-at-120-or-inarnold-for-3-consecutive-days-notethe-prepared-culture-media-should-be-carefullystored-in-a-dark-cool-place-if-they-are-to-be-kept-for-a-consid-preparation-of-culture-media-29-erable-length-of-time-they-should-be-sealed-either-with-paraffinor-with-a-rubber-cap-th-image370176452.htmlRM2CE6YRG–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . Fig. 17Ordinary Style of Potato-Tubeu.. Potato 4. Cotton 2. Add 7-10 per cent, of a solution of litmus (tinctureof litmus). 3. Distribute about 7 c.c. into each of twenty-five tubes. 4. Sterilize in autoclav for 5 minutes at 120°, or inArnold for 3 consecutive days. Note.—The prepared culture media should be carefullystored in a dark, cool place. If they are to be kept for a consid- PREPARATION OF CULTURE MEDIA 29 erable length of time, they should be sealed either with paraffinor with a rubber cap. Th
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. at the boiling point filter the agar and dis-tribute into culture tubes. 9. The tubed agar should be sterilized in the auto-clave for 5 minutes. For agar slants each tube should contain about 7 c.c.or be filled one-third of the length of the tube. Forplating, the tube should be half filled and contain 10c.c. Slants are prepared by allowing the agar aftersterihzation to cool in a slanting position. If it is de-sired to slant a large number a whole basket may beput in a slanting position. When a few tubes Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-at-the-boiling-point-filter-the-agar-and-dis-tribute-into-culture-tubes-9-the-tubed-agar-should-be-sterilized-in-the-auto-clave-for-5-minutes-for-agar-slants-each-tube-should-contain-about-7-ccor-be-filled-one-third-of-the-length-of-the-tube-forplating-the-tube-should-be-half-filled-and-contain-10cc-slants-are-prepared-by-allowing-the-agar-aftersterihzation-to-cool-in-a-slanting-position-if-it-is-de-sired-to-slant-a-large-number-a-whole-basket-may-beput-in-a-slanting-position-when-a-few-tubes-image370322981.htmlRM2CEDJMN–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. at the boiling point filter the agar and dis-tribute into culture tubes. 9. The tubed agar should be sterilized in the auto-clave for 5 minutes. For agar slants each tube should contain about 7 c.c.or be filled one-third of the length of the tube. Forplating, the tube should be half filled and contain 10c.c. Slants are prepared by allowing the agar aftersterihzation to cool in a slanting position. If it is de-sired to slant a large number a whole basket may beput in a slanting position. When a few tubes
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Fig. 17.—Sterilized test-tubeand swab for collecting pus andfluids for bacteriological examina-tion (Warren). Fig. 18.—Apparatus for the collec-tion of pathological fluids. well covered. This is placed, cotton end first, in a test-tube, which is then provided with a cotton stopper (Fig.17), and the whole sterilized in a hot-air sterilizer by heat-ing to 1500 to 1800 C. during about half an hour. A largenumber of swabs in test-tubes may be kept on hand ster-ilized and ready for use. CULTURE METH Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathological-technique-a-practical-manual-for-workers-in-pathological-histology-and-bacteriology-fig-17sterilized-test-tubeand-swab-for-collecting-pus-andfluids-for-bacteriological-examina-tion-warren-fig-18apparatus-for-the-collec-tion-of-pathological-fluids-well-covered-this-is-placed-cotton-end-first-in-a-test-tube-which-is-then-provided-with-a-cotton-stopper-fig17-and-the-whole-sterilized-in-a-hot-air-sterilizer-by-heat-ing-to-1500-to-1800-c-during-about-half-an-hour-a-largenumber-of-swabs-in-test-tubes-may-be-kept-on-hand-ster-ilized-and-ready-for-use-culture-meth-image370419929.htmlRM2CEJ2B5–. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Fig. 17.—Sterilized test-tubeand swab for collecting pus andfluids for bacteriological examina-tion (Warren). Fig. 18.—Apparatus for the collec-tion of pathological fluids. well covered. This is placed, cotton end first, in a test-tube, which is then provided with a cotton stopper (Fig.17), and the whole sterilized in a hot-air sterilizer by heat-ing to 1500 to 1800 C. during about half an hour. A largenumber of swabs in test-tubes may be kept on hand ster-ilized and ready for use. CULTURE METH
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. vy w Fig. IIKoch Inspissator 1. Close the Ud and the steam outlet. 2. Admit steam. After reaching 3 pqunds pressure,keep this pressure for 5 minutes. 3. Increase the pressure slowly to 5 pounds andkeep there for 5 minutes. 18 LABORATORY GUIDE EST BACTERIOLOGY 4. Increase to 10 pounds and hold for 5 minutes. 5. Increase to 15 pounds and hold for 5 minutes. 6. Open the steam outlet and keep at 15 poimdspressure for 20 minutes. It is advisable to sterilize the tubes again on thefollowing day by slowly brin Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-vy-w-fig-iikoch-inspissator-1-close-the-ud-and-the-steam-outlet-2-admit-steam-after-reaching-3-pqunds-pressurekeep-this-pressure-for-5-minutes-3-increase-the-pressure-slowly-to-5-pounds-andkeep-there-for-5-minutes-18-laboratory-guide-est-bacteriology-4-increase-to-10-pounds-and-hold-for-5-minutes-5-increase-to-15-pounds-and-hold-for-5-minutes-6-open-the-steam-outlet-and-keep-at-15-poimdspressure-for-20-minutes-it-is-advisable-to-sterilize-the-tubes-again-on-thefollowing-day-by-slowly-brin-image370322931.htmlRM2CEDJJY–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. vy w Fig. IIKoch Inspissator 1. Close the Ud and the steam outlet. 2. Admit steam. After reaching 3 pqunds pressure,keep this pressure for 5 minutes. 3. Increase the pressure slowly to 5 pounds andkeep there for 5 minutes. 18 LABORATORY GUIDE EST BACTERIOLOGY 4. Increase to 10 pounds and hold for 5 minutes. 5. Increase to 15 pounds and hold for 5 minutes. 6. Open the steam outlet and keep at 15 poimdspressure for 20 minutes. It is advisable to sterilize the tubes again on thefollowing day by slowly brin
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. to directions. 4. Store cover sUps in a stender dish and coverthem with alcohol. A soft linen cloth should be usedfor cleaning. The following outfit will be needed for each course.Additions to this outfit will be designated as requiredin the respective courses. 200 culture tubes. 20 potato tubes. 12 fermentation tubes. 20 petri dishes. 3 Erlenmeyer flasks, one 1,000 c.c, two 500 c.c. each. 2 glass funnels, one 4 inches, one 6 inches. 4 bottles for staining fluids.I balsam bottle. I stender dish. 3 stain Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-to-directions-4-store-cover-sups-in-a-stender-dish-and-coverthem-with-alcohol-a-soft-linen-cloth-should-be-usedfor-cleaning-the-following-outfit-will-be-needed-for-each-courseadditions-to-this-outfit-will-be-designated-as-requiredin-the-respective-courses-200-culture-tubes-20-potato-tubes-12-fermentation-tubes-20-petri-dishes-3-erlenmeyer-flasks-one-1000-cc-two-500-cc-each-2-glass-funnels-one-4-inches-one-6-inches-4-bottles-for-staining-fluidsi-balsam-bottle-i-stender-dish-3-stain-image370323847.htmlRM2CEDKRK–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. to directions. 4. Store cover sUps in a stender dish and coverthem with alcohol. A soft linen cloth should be usedfor cleaning. The following outfit will be needed for each course.Additions to this outfit will be designated as requiredin the respective courses. 200 culture tubes. 20 potato tubes. 12 fermentation tubes. 20 petri dishes. 3 Erlenmeyer flasks, one 1,000 c.c, two 500 c.c. each. 2 glass funnels, one 4 inches, one 6 inches. 4 bottles for staining fluids.I balsam bottle. I stender dish. 3 stain
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. ther media later. 69 70 LABORATORY GUIDE IN BACTERIOLOGY SECTION 2 COLLECTING AND CULTIVATING MICRO-ORGANISMSFROM THE AIR EXERCISE I 1. Sterilize all petri dishes in the hot-air sterilizer forone hour at i6o° C. 2. Melt two tubes of plain agar, one of dextroseagar, and two.of beerwort agar, in a water bath. Thewater bath (Fig. 25) is a round copper vessel with anumber of holes in the top. These holes are largeenough to allow a culture tube to slip in. A thermom-eter is passed through a rubber cork with Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-ther-media-later-69-70-laboratory-guide-in-bacteriology-section-2-collecting-and-cultivating-micro-organismsfrom-the-air-exercise-i-1-sterilize-all-petri-dishes-in-the-hot-air-sterilizer-forone-hour-at-i6o-c-2-melt-two-tubes-of-plain-agar-one-of-dextroseagar-and-twoof-beerwort-agar-in-a-water-bath-thewater-bath-fig-25-is-a-round-copper-vessel-with-anumber-of-holes-in-the-top-these-holes-are-largeenough-to-allow-a-culture-tube-to-slip-in-a-thermom-eter-is-passed-through-a-rubber-cork-with-image370322177.htmlRM2CEDHM1–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. ther media later. 69 70 LABORATORY GUIDE IN BACTERIOLOGY SECTION 2 COLLECTING AND CULTIVATING MICRO-ORGANISMSFROM THE AIR EXERCISE I 1. Sterilize all petri dishes in the hot-air sterilizer forone hour at i6o° C. 2. Melt two tubes of plain agar, one of dextroseagar, and two.of beerwort agar, in a water bath. Thewater bath (Fig. 25) is a round copper vessel with anumber of holes in the top. These holes are largeenough to allow a culture tube to slip in. A thermom-eter is passed through a rubber cork with
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . ary type deposit10 g. of pyrogaUic acid. b) Smear vaseUn around the mouth of the jar. c) Pour into the jar 100 c.c. of a i per cent, solutionof NaOH. d) Then deposit in the jar culture-tubes previouslyinoculated. e) Tightly fasten the cover of the jar, and incubateat 37° for 48-72 hours. 4. Special study D.—Cultivation in hydrogen gas. a) Inoculate all media from stock-cultures obtained. b) Fit up apparatus as shown in Fig. 34. c) Place culture-tubes in a Novy jar (Fig. 34, a). ANAEROBIC GROUP OF BACIL Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-ary-type-deposit10-g-of-pyrogauic-acid-b-smear-vaseun-around-the-mouth-of-the-jar-c-pour-into-the-jar-100-cc-of-a-i-per-cent-solutionof-naoh-d-then-deposit-in-the-jar-culture-tubes-previouslyinoculated-e-tightly-fasten-the-cover-of-the-jar-and-incubateat-37-for-48-72-hours-4-special-study-dcultivation-in-hydrogen-gas-a-inoculate-all-media-from-stock-cultures-obtained-b-fit-up-apparatus-as-shown-in-fig-34-c-place-culture-tubes-in-a-novy-jar-fig-34-a-anaerobic-group-of-bacil-image370175953.htmlRM2CE6Y5N–. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . ary type deposit10 g. of pyrogaUic acid. b) Smear vaseUn around the mouth of the jar. c) Pour into the jar 100 c.c. of a i per cent, solutionof NaOH. d) Then deposit in the jar culture-tubes previouslyinoculated. e) Tightly fasten the cover of the jar, and incubateat 37° for 48-72 hours. 4. Special study D.—Cultivation in hydrogen gas. a) Inoculate all media from stock-cultures obtained. b) Fit up apparatus as shown in Fig. 34. c) Place culture-tubes in a Novy jar (Fig. 34, a). ANAEROBIC GROUP OF BACIL
. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. 84 PHARMACEUTICAL BACTERIOLOGY h. Beep stab culture—where is growth most active? If at bottom, it indicates anaerobic tendencies. If limited to top of medium, it indi- cates decidedly aerobic tendencies. (Most bacteria are decidedly aerobic; that is, they require free oxygen to thrive.) The test-tube cultures do not necessarily represent pure cultures, and the student cannot know whether the growths in the test-tubes represent the predominating bacterial flora in the substance from which the inoculations were made. Th Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pharmaceutical-bacteriology-bacteriology-disinfection-and-disinfectants-84-pharmaceutical-bacteriology-h-beep-stab-culturewhere-is-growth-most-active-if-at-bottom-it-indicates-anaerobic-tendencies-if-limited-to-top-of-medium-it-indi-cates-decidedly-aerobic-tendencies-most-bacteria-are-decidedly-aerobic-that-is-they-require-free-oxygen-to-thrive-the-test-tube-cultures-do-not-necessarily-represent-pure-cultures-and-the-student-cannot-know-whether-the-growths-in-the-test-tubes-represent-the-predominating-bacterial-flora-in-the-substance-from-which-the-inoculations-were-made-th-image232443650.htmlRMRE4M6X–. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. 84 PHARMACEUTICAL BACTERIOLOGY h. Beep stab culture—where is growth most active? If at bottom, it indicates anaerobic tendencies. If limited to top of medium, it indi- cates decidedly aerobic tendencies. (Most bacteria are decidedly aerobic; that is, they require free oxygen to thrive.) The test-tube cultures do not necessarily represent pure cultures, and the student cannot know whether the growths in the test-tubes represent the predominating bacterial flora in the substance from which the inoculations were made. Th
. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. STERILIZATION—ANTISEPSIS—FOOD PRESERVATION 67 above the surface of the water is for the reception of the tubes and flasks that are to be steriUzed. The Arnold steam steril- izer is somewhat more complicated but is very convenient and efficient. It consists of a cylinder of tin or copper with a cover, which is enclosed in a movable cylindrical outer cover or hood. The inner cylinder has an opening in the bottom through which s Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathogenic-micro-organisms-a-text-book-of-microbiology-for-physicians-and-students-of-medicine-based-upon-williams-bacteriology-bacteriology-pathogenic-bacteria-sterilizationantisepsisfood-preservation-67-above-the-surface-of-the-water-is-for-the-reception-of-the-tubes-and-flasks-that-are-to-be-steriuzed-the-arnold-steam-steril-izer-is-somewhat-more-complicated-but-is-very-convenient-and-efficient-it-consists-of-a-cylinder-of-tin-or-copper-with-a-cover-which-is-enclosed-in-a-movable-cylindrical-outer-cover-or-hood-the-inner-cylinder-has-an-opening-in-the-bottom-through-which-s-image232419565.htmlRMRE3HEN–. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. STERILIZATION—ANTISEPSIS—FOOD PRESERVATION 67 above the surface of the water is for the reception of the tubes and flasks that are to be steriUzed. The Arnold steam steril- izer is somewhat more complicated but is very convenient and efficient. It consists of a cylinder of tin or copper with a cover, which is enclosed in a movable cylindrical outer cover or hood. The inner cylinder has an opening in the bottom through which s
. Bacteriology and mycology of foods. Food. 128 STERILIZATION AND DISINFECTION and unchanged. Body extracts and sera may be thus treated to render them sterile. Filtration through Liquids. The action is entiiely mechanical unless some strong hquid such as sodmm hydroxide or sulphuric acid are used. In these cases the bacterial protein is decomposed by the chemical. Rettger appUed this method to the bacterial examination of air. Filtration through Solids. The same principle is involved here that is involved in the use of the cotton plug in test tubes and culture flasks. Different substances hav Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriology-and-mycology-of-foods-food-128-sterilization-and-disinfection-and-unchanged-body-extracts-and-sera-may-be-thus-treated-to-render-them-sterile-filtration-through-liquids-the-action-is-entiiely-mechanical-unless-some-strong-hquid-such-as-sodmm-hydroxide-or-sulphuric-acid-are-used-in-these-cases-the-bacterial-protein-is-decomposed-by-the-chemical-rettger-appued-this-method-to-the-bacterial-examination-of-air-filtration-through-solids-the-same-principle-is-involved-here-that-is-involved-in-the-use-of-the-cotton-plug-in-test-tubes-and-culture-flasks-different-substances-hav-image231892110.htmlRMRD7GN2–. Bacteriology and mycology of foods. Food. 128 STERILIZATION AND DISINFECTION and unchanged. Body extracts and sera may be thus treated to render them sterile. Filtration through Liquids. The action is entiiely mechanical unless some strong hquid such as sodmm hydroxide or sulphuric acid are used. In these cases the bacterial protein is decomposed by the chemical. Rettger appUed this method to the bacterial examination of air. Filtration through Solids. The same principle is involved here that is involved in the use of the cotton plug in test tubes and culture flasks. Different substances hav
. A laboratory guide in bacteriology, for the use of students, teachers and practitioners. Bacteriology. 10 LABORATORY GUIDE IN BACTERIOLOGY at a temperature of 150° C. for about 30 minutes, or until the plugs are slightly browned. The tubes are not necessarily sterile, but the plugs have become set so as to fit the mouth of the tube, and may be removed and replaced readily. SECTION 4 METHODS OF STERILIZATION Sterilization is the process of removing all living organisms. This may be accomplished by heat, by. Fig. 3 Berkefeld Filter o. Berkefeld filter d. Intercepting flask b. Filtered liquid e Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-guide-in-bacteriology-for-the-use-of-students-teachers-and-practitioners-bacteriology-10-laboratory-guide-in-bacteriology-at-a-temperature-of-150-c-for-about-30-minutes-or-until-the-plugs-are-slightly-browned-the-tubes-are-not-necessarily-sterile-but-the-plugs-have-become-set-so-as-to-fit-the-mouth-of-the-tube-and-may-be-removed-and-replaced-readily-section-4-methods-of-sterilization-sterilization-is-the-process-of-removing-all-living-organisms-this-may-be-accomplished-by-heat-by-fig-3-berkefeld-filter-o-berkefeld-filter-d-intercepting-flask-b-filtered-liquid-e-image232444079.htmlRMRE4MP7–. A laboratory guide in bacteriology, for the use of students, teachers and practitioners. Bacteriology. 10 LABORATORY GUIDE IN BACTERIOLOGY at a temperature of 150° C. for about 30 minutes, or until the plugs are slightly browned. The tubes are not necessarily sterile, but the plugs have become set so as to fit the mouth of the tube, and may be removed and replaced readily. SECTION 4 METHODS OF STERILIZATION Sterilization is the process of removing all living organisms. This may be accomplished by heat, by. Fig. 3 Berkefeld Filter o. Berkefeld filter d. Intercepting flask b. Filtered liquid e
. Bacteriological apparatus : pathological, biochemical. Scientific apparatus and instruments; Bacteriology; Chemical industry. 17720 17725 17720 Support—Nessler Tube. With base covered by a milk-glass plate upon which the tubes rest Each 2.50 17725 Support. Attachable to wall or table; fitted with rod Each .60. 17738. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Will Corporation. Rochester, N. Y. : The Will Corporation Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-apparatus-pathological-biochemical-scientific-apparatus-and-instruments-bacteriology-chemical-industry-17720-17725-17720-supportnessler-tube-with-base-covered-by-a-milk-glass-plate-upon-which-the-tubes-rest-each-250-17725-support-attachable-to-wall-or-table-fitted-with-rod-each-60-17738-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-digitally-enhanced-for-readability-coloration-and-appearance-of-these-illustrations-may-not-perfectly-resemble-the-original-work-will-corporation-rochester-n-y-the-will-corporation-image235258318.htmlRMRJMXAP–. Bacteriological apparatus : pathological, biochemical. Scientific apparatus and instruments; Bacteriology; Chemical industry. 17720 17725 17720 Support—Nessler Tube. With base covered by a milk-glass plate upon which the tubes rest Each 2.50 17725 Support. Attachable to wall or table; fitted with rod Each .60. 17738. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Will Corporation. Rochester, N. Y. : The Will Corporation
. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. Fig . 26.—^Hot water funnel with stand and ring gas burner. Fig. 27.—Hot water funnel with stand. tube slant. The swab is returned to the tube, the cotton plug is restored and then returned to the board of health to be destroyed in stove or furnace fire, or destroyed by the attending physician in case there is no board of health to recieve it. Fig. 28.—Glass rods with platinum wire, straight and loop, for inoculating culture tubes, Petri plates, etc.—(WiUiams.) 7. Making Bacterial Cultures. T Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pharmaceutical-bacteriology-with-special-reference-to-disinfection-and-sterilization-bacteriology-fig-26hot-water-funnel-with-stand-and-ring-gas-burner-fig-27hot-water-funnel-with-stand-tube-slant-the-swab-is-returned-to-the-tube-the-cotton-plug-is-restored-and-then-returned-to-the-board-of-health-to-be-destroyed-in-stove-or-furnace-fire-or-destroyed-by-the-attending-physician-in-case-there-is-no-board-of-health-to-recieve-it-fig-28glass-rods-with-platinum-wire-straight-and-loop-for-inoculating-culture-tubes-petri-plates-etcwiuiams-7-making-bacterial-cultures-t-image232385959.htmlRMRE22JF–. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. Fig . 26.—^Hot water funnel with stand and ring gas burner. Fig. 27.—Hot water funnel with stand. tube slant. The swab is returned to the tube, the cotton plug is restored and then returned to the board of health to be destroyed in stove or furnace fire, or destroyed by the attending physician in case there is no board of health to recieve it. Fig. 28.—Glass rods with platinum wire, straight and loop, for inoculating culture tubes, Petri plates, etc.—(WiUiams.) 7. Making Bacterial Cultures. T
. A text-book of bacteriology. Bacteriology. STERILIZATION OF CULTURE MEDIA. 61 receptacle, R, is provided for the liquid to be filtered, and a pump for compressing air is attached to it by a rubber tube. Instead of this pump, water pressure may be used indirectly by attaching a strong bottle to the water supply and allowing it to fill slowly with water, and at the same time to force out the air through a tube connected with the filtering apparatus. For this purpose the bottle, having a capacity of a quart or more, should be provided with a rubber stop- per through which two short tubes are pa Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-bacteriology-bacteriology-sterilization-of-culture-media-61-receptacle-r-is-provided-for-the-liquid-to-be-filtered-and-a-pump-for-compressing-air-is-attached-to-it-by-a-rubber-tube-instead-of-this-pump-water-pressure-may-be-used-indirectly-by-attaching-a-strong-bottle-to-the-water-supply-and-allowing-it-to-fill-slowly-with-water-and-at-the-same-time-to-force-out-the-air-through-a-tube-connected-with-the-filtering-apparatus-for-this-purpose-the-bottle-having-a-capacity-of-a-quart-or-more-should-be-provided-with-a-rubber-stop-per-through-which-two-short-tubes-are-pa-image232443759.htmlRMRE4MAR–. A text-book of bacteriology. Bacteriology. STERILIZATION OF CULTURE MEDIA. 61 receptacle, R, is provided for the liquid to be filtered, and a pump for compressing air is attached to it by a rubber tube. Instead of this pump, water pressure may be used indirectly by attaching a strong bottle to the water supply and allowing it to fill slowly with water, and at the same time to force out the air through a tube connected with the filtering apparatus. For this purpose the bottle, having a capacity of a quart or more, should be provided with a rubber stop- per through which two short tubes are pa
. A manual of bacteriology. Bacteriology. PATHOGENIC BACTERIA. 341 Bacteriological Diagnosis oj Diphtheria.—In many large cities the bacteriological diagnosis of diphtheria is undertaken by boards of health. The methods used differ somewhat in detail, but are similar in the main, and are based upon the procedure devised by Biggs and Pack for the Board of Health of New York City. Two tubes are furnished in a box. The tubes are like ordinary test-tubes, about three inches in length, rather heavy and without a flange. Both are plugged with cotton. One contains slanted and sterilized LofHer's bloo Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-manual-of-bacteriology-bacteriology-pathogenic-bacteria-341-bacteriological-diagnosis-oj-diphtheriain-many-large-cities-the-bacteriological-diagnosis-of-diphtheria-is-undertaken-by-boards-of-health-the-methods-used-differ-somewhat-in-detail-but-are-similar-in-the-main-and-are-based-upon-the-procedure-devised-by-biggs-and-pack-for-the-board-of-health-of-new-york-city-two-tubes-are-furnished-in-a-box-the-tubes-are-like-ordinary-test-tubes-about-three-inches-in-length-rather-heavy-and-without-a-flange-both-are-plugged-with-cotton-one-contains-slanted-and-sterilized-lofhers-bloo-image232372270.htmlRMRE1D5J–. A manual of bacteriology. Bacteriology. PATHOGENIC BACTERIA. 341 Bacteriological Diagnosis oj Diphtheria.—In many large cities the bacteriological diagnosis of diphtheria is undertaken by boards of health. The methods used differ somewhat in detail, but are similar in the main, and are based upon the procedure devised by Biggs and Pack for the Board of Health of New York City. Two tubes are furnished in a box. The tubes are like ordinary test-tubes, about three inches in length, rather heavy and without a flange. Both are plugged with cotton. One contains slanted and sterilized LofHer's bloo
. A laboratory manual of soil bacteriology. Soil microbiology. SPECIAL METHODS 159 necessary. Although there are a great number of vessels designed for this purpose, only one will be described. It is obvious from the start that apparatus of this nature must be somewhat complicated. A modification of the Schulze and Schulow methods has been found fairly satis- factory. Apparatus: One large Woulfe's bottle, about 3-liter capacity, with three openings. One large 2- or 3-litcr flask, Erlcnmeyer form. Two U tubes. One cylinder constructed as shown in Fig. 13. The apparatus is arranged according to Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-laboratory-manual-of-soil-bacteriology-soil-microbiology-special-methods-159-necessary-although-there-are-a-great-number-of-vessels-designed-for-this-purpose-only-one-will-be-described-it-is-obvious-from-the-start-that-apparatus-of-this-nature-must-be-somewhat-complicated-a-modification-of-the-schulze-and-schulow-methods-has-been-found-fairly-satis-factory-apparatus-one-large-woulfes-bottle-about-3-liter-capacity-with-three-openings-one-large-2-or-3-litcr-flask-erlcnmeyer-form-two-u-tubes-one-cylinder-constructed-as-shown-in-fig-13-the-apparatus-is-arranged-according-to-image231921535.htmlRMRD8X7Y–. A laboratory manual of soil bacteriology. Soil microbiology. SPECIAL METHODS 159 necessary. Although there are a great number of vessels designed for this purpose, only one will be described. It is obvious from the start that apparatus of this nature must be somewhat complicated. A modification of the Schulze and Schulow methods has been found fairly satis- factory. Apparatus: One large Woulfe's bottle, about 3-liter capacity, with three openings. One large 2- or 3-litcr flask, Erlcnmeyer form. Two U tubes. One cylinder constructed as shown in Fig. 13. The apparatus is arranged according to
. A text-book of bacteriology. Bacteriology. NOT DESCRIBED IN PREVIOUS SECTIONS. 639 My notes indicate that cultures passed through the guinea-pig are more apt to be motile. In gelatin stab cultures the growth of bacillus x resembles that of the â¢colon bacillus, but the colonies at the bottom of the line of puncture are more opaque and not of a clear amber color like that of colonies of the colon bacillus. Upon the surface the growth is thicker than that of the colon bacillus, and forms a milk-white, soft mass. The colonies in gelatin Esmarch roll tubes vary considerably at different times. D Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-bacteriology-bacteriology-not-described-in-previous-sections-639-my-notes-indicate-that-cultures-passed-through-the-guinea-pig-are-more-apt-to-be-motile-in-gelatin-stab-cultures-the-growth-of-bacillus-x-resembles-that-of-the-colon-bacillus-but-the-colonies-at-the-bottom-of-the-line-of-puncture-are-more-opaque-and-not-of-a-clear-amber-color-like-that-of-colonies-of-the-colon-bacillus-upon-the-surface-the-growth-is-thicker-than-that-of-the-colon-bacillus-and-forms-a-milk-white-soft-mass-the-colonies-in-gelatin-esmarch-roll-tubes-vary-considerably-at-different-times-d-image232385830.htmlRMRE22DX–. A text-book of bacteriology. Bacteriology. NOT DESCRIBED IN PREVIOUS SECTIONS. 639 My notes indicate that cultures passed through the guinea-pig are more apt to be motile. In gelatin stab cultures the growth of bacillus x resembles that of the â¢colon bacillus, but the colonies at the bottom of the line of puncture are more opaque and not of a clear amber color like that of colonies of the colon bacillus. Upon the surface the growth is thicker than that of the colon bacillus, and forms a milk-white, soft mass. The colonies in gelatin Esmarch roll tubes vary considerably at different times. D
. Bacteria in relation to plant diseases. Bacteriology; Plant diseases. 98 BACTERIA IN RELATION TO PLANT DISEASES. mountain regions must itse autoclaves. Agar, potato, etc., in test-tiibes, may be steamed twenty minutes on eacli of three consecutive days. Gelatin, beef-bouillon, and all other fluids likel}' to be injured by long heating should be steamed only ten or fifteen minutes on each of three consecutive days, if in tubes. The writer frequently steams such media fifteen minutes the first day, ten minutes the second, and five minutes the third. Agar, gelatin, bouillon, etc., stored in fla Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteria-in-relation-to-plant-diseases-bacteriology-plant-diseases-98-bacteria-in-relation-to-plant-diseases-mountain-regions-must-itse-autoclaves-agar-potato-etc-in-test-tiibes-may-be-steamed-twenty-minutes-on-eacli-of-three-consecutive-days-gelatin-beef-bouillon-and-all-other-fluids-likel-to-be-injured-by-long-heating-should-be-steamed-only-ten-or-fifteen-minutes-on-each-of-three-consecutive-days-if-in-tubes-the-writer-frequently-steams-such-media-fifteen-minutes-the-first-day-ten-minutes-the-second-and-five-minutes-the-third-agar-gelatin-bouillon-etc-stored-in-fla-image232236889.htmlRMRDR8EH–. Bacteria in relation to plant diseases. Bacteriology; Plant diseases. 98 BACTERIA IN RELATION TO PLANT DISEASES. mountain regions must itse autoclaves. Agar, potato, etc., in test-tiibes, may be steamed twenty minutes on eacli of three consecutive days. Gelatin, beef-bouillon, and all other fluids likel}' to be injured by long heating should be steamed only ten or fifteen minutes on each of three consecutive days, if in tubes. The writer frequently steams such media fifteen minutes the first day, ten minutes the second, and five minutes the third. Agar, gelatin, bouillon, etc., stored in fla
. Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. D Petri Dishes. These should be about 10 cm. in diameter, thus having an area of about 65 sq. cm. Either glass or porous tops may be used. The porous tops devised by Hill prevent the spreading colonies which appear so often on Petri dishes in humid weather. Very often it is difficult to secure Petri dishes with flat bottoms. This is essential to an even distribution of bacteria over the plate and a unif Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriology-and-mycology-of-foods-food-4l-a-fig-1-types-of-fermentation-tubes-af-browns-j5-smiths-c-smiths-without-foot-d-graves-modification-of-the-durham-fermentation-tube-d-petri-dishes-these-should-be-about-10-cm-in-diameter-thus-having-an-area-of-about-65-sq-cm-either-glass-or-porous-tops-may-be-used-the-porous-tops-devised-by-hill-prevent-the-spreading-colonies-which-appear-so-often-on-petri-dishes-in-humid-weather-very-often-it-is-difficult-to-secure-petri-dishes-with-flat-bottoms-this-is-essential-to-an-even-distribution-of-bacteria-over-the-plate-and-a-unif-image231920877.htmlRMRD8WCD–. Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. D Petri Dishes. These should be about 10 cm. in diameter, thus having an area of about 65 sq. cm. Either glass or porous tops may be used. The porous tops devised by Hill prevent the spreading colonies which appear so often on Petri dishes in humid weather. Very often it is difficult to secure Petri dishes with flat bottoms. This is essential to an even distribution of bacteria over the plate and a unif
. Bacteria in relation to soil fertility. Soil microbiology; Bacteriology, Agricultural. THE CHEMISTRY OF BACTERIA 43 required for the growth of different bacteria just as various higher plants require different soils. Most bacteria grow well in beef tea. However, we must prevent the organisms from swim- ming about and thus mixing during growth. This is accom- plished by adding to the medium one per cent of a seaweed, agar- agar. Such a medium is placed in large flasks, in test tubes stop- pered with cotton plugs, or shallow dishes covered with a second. Fig. 21.—Sterilizers used in bacteriolo Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteria-in-relation-to-soil-fertility-soil-microbiology-bacteriology-agricultural-the-chemistry-of-bacteria-43-required-for-the-growth-of-different-bacteria-just-as-various-higher-plants-require-different-soils-most-bacteria-grow-well-in-beef-tea-however-we-must-prevent-the-organisms-from-swim-ming-about-and-thus-mixing-during-growth-this-is-accom-plished-by-adding-to-the-medium-one-per-cent-of-a-seaweed-agar-agar-such-a-medium-is-placed-in-large-flasks-in-test-tubes-stop-pered-with-cotton-plugs-or-shallow-dishes-covered-with-a-second-fig-21sterilizers-used-in-bacteriolo-image231921341.htmlRMRD8X11–. Bacteria in relation to soil fertility. Soil microbiology; Bacteriology, Agricultural. THE CHEMISTRY OF BACTERIA 43 required for the growth of different bacteria just as various higher plants require different soils. Most bacteria grow well in beef tea. However, we must prevent the organisms from swim- ming about and thus mixing during growth. This is accom- plished by adding to the medium one per cent of a seaweed, agar- agar. Such a medium is placed in large flasks, in test tubes stop- pered with cotton plugs, or shallow dishes covered with a second. Fig. 21.—Sterilizers used in bacteriolo
. A text-book of bacteriology. Bacteriology. CULTURES IN LIQUID MEDIA. 63 For larger quantities of liquid than can be held in an ordinary test tube ihe small flasks with a flat bottom, known as Erlenmeyer flasks, are very convenient (Fig. 33). In his earlier researches Pasteur used flasks and tubes of various forms, which served a useful purpose, but have been displaced in his laboratory by the simpler form of apparatus shown in Fig. 34. This is a httle flask having a cover which is ground to fit the neck. This cover is drawn out above into a narrow tube which admits oxygen to the flask throug Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-bacteriology-bacteriology-cultures-in-liquid-media-63-for-larger-quantities-of-liquid-than-can-be-held-in-an-ordinary-test-tube-ihe-small-flasks-with-a-flat-bottom-known-as-erlenmeyer-flasks-are-very-convenient-fig-33-in-his-earlier-researches-pasteur-used-flasks-and-tubes-of-various-forms-which-served-a-useful-purpose-but-have-been-displaced-in-his-laboratory-by-the-simpler-form-of-apparatus-shown-in-fig-34-this-is-a-httle-flask-having-a-cover-which-is-ground-to-fit-the-neck-this-cover-is-drawn-out-above-into-a-narrow-tube-which-admits-oxygen-to-the-flask-throug-image232443755.htmlRMRE4MAK–. A text-book of bacteriology. Bacteriology. CULTURES IN LIQUID MEDIA. 63 For larger quantities of liquid than can be held in an ordinary test tube ihe small flasks with a flat bottom, known as Erlenmeyer flasks, are very convenient (Fig. 33). In his earlier researches Pasteur used flasks and tubes of various forms, which served a useful purpose, but have been displaced in his laboratory by the simpler form of apparatus shown in Fig. 34. This is a httle flask having a cover which is ground to fit the neck. This cover is drawn out above into a narrow tube which admits oxygen to the flask throug
. Manual of bacteriology. Bacteriology. 6o METHODS OF CULTIVATION OF BACTERIA.. Fig. 24. — Esmarch's roll-tube adapted for cul- ture containing anaerobes. ing from the last bottle to the vessel containing the medium ought to be sterilised by passing through a Bunsen flame, and should have a small plug of cotton wool in it to filter the hydro- gen germ-free. Separation of Anaerobic Organisms. — {a) By Roll-tubes. — A i^ inch test-tube has as much gelatin put into it as would be ., I used in the Esmarch roll-tube method. It is corked with an india-rubber stopper hav- ing two tubes passing throug Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/manual-of-bacteriology-bacteriology-6o-methods-of-cultivation-of-bacteria-fig-24-esmarchs-roll-tube-adapted-for-cul-ture-containing-anaerobes-ing-from-the-last-bottle-to-the-vessel-containing-the-medium-ought-to-be-sterilised-by-passing-through-a-bunsen-flame-and-should-have-a-small-plug-of-cotton-wool-in-it-to-filter-the-hydro-gen-germ-free-separation-of-anaerobic-organisms-a-by-roll-tubes-a-i-inch-test-tube-has-as-much-gelatin-put-into-it-as-would-be-i-used-in-the-esmarch-roll-tube-method-it-is-corked-with-an-india-rubber-stopper-hav-ing-two-tubes-passing-throug-image232443909.htmlRMRE4MG5–. Manual of bacteriology. Bacteriology. 6o METHODS OF CULTIVATION OF BACTERIA.. Fig. 24. — Esmarch's roll-tube adapted for cul- ture containing anaerobes. ing from the last bottle to the vessel containing the medium ought to be sterilised by passing through a Bunsen flame, and should have a small plug of cotton wool in it to filter the hydro- gen germ-free. Separation of Anaerobic Organisms. — {a) By Roll-tubes. — A i^ inch test-tube has as much gelatin put into it as would be ., I used in the Esmarch roll-tube method. It is corked with an india-rubber stopper hav- ing two tubes passing throug
. Bacteriological apparatus : pathological, biochemical. Scientific apparatus and instruments; Bacteriology; Chemical industry. 14970 / 14985 15005 15020 14965 Creamometer. To show percentage of cream formed on milk; con- sists of six graduated glass tubes, supported on wooden stand Each 3.50 14970 Cylinders. Creamometer jars. No. A B Graduated to 0-30 0-90 Each .52 .56 14975 Lactobutyrometer—Caldwell's. For determining amount of fat in milk; consists of graduated tube with rubber stopper, two volume pipettes and one cylinder jar Each 2.50 14980 Lactobutyrometer—Marchand's. For fat determinat Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-apparatus-pathological-biochemical-scientific-apparatus-and-instruments-bacteriology-chemical-industry-14970-14985-15005-15020-14965-creamometer-to-show-percentage-of-cream-formed-on-milk-con-sists-of-six-graduated-glass-tubes-supported-on-wooden-stand-each-350-14970-cylinders-creamometer-jars-no-a-b-graduated-to-0-30-0-90-each-52-56-14975-lactobutyrometercaldwells-for-determining-amount-of-fat-in-milk-consists-of-graduated-tube-with-rubber-stopper-two-volume-pipettes-and-one-cylinder-jar-each-250-14980-lactobutyrometermarchands-for-fat-determinat-image235259293.htmlRMRJMYHH–. Bacteriological apparatus : pathological, biochemical. Scientific apparatus and instruments; Bacteriology; Chemical industry. 14970 / 14985 15005 15020 14965 Creamometer. To show percentage of cream formed on milk; con- sists of six graduated glass tubes, supported on wooden stand Each 3.50 14970 Cylinders. Creamometer jars. No. A B Graduated to 0-30 0-90 Each .52 .56 14975 Lactobutyrometer—Caldwell's. For determining amount of fat in milk; consists of graduated tube with rubber stopper, two volume pipettes and one cylinder jar Each 2.50 14980 Lactobutyrometer—Marchand's. For fat determinat
. Bacteria in relation to plant diseases. Bacteriology; Plant diseases. INDOL, PHENOL, ETC. 6:. d for five minutes (70° to 80° C). The browniiifj of media due to excess of sodiuiu nitrite must not be mistaken for this pink or red reaction. Uninoculated tubes should be inchided in the test, wliich may be made on the second and tenth day. For methods of determining phenol see Lewandowski in Deutsche Med. Wochenschrift, 1890, p. 1186, and Chester's Manual, p. t,;},. Schmidt (Bd. II, p. 1008) gives the following as a qualitative reaction for tyrosiu : Dissolve !:>}' boiling in water and add a s Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteria-in-relation-to-plant-diseases-bacteriology-plant-diseases-indol-phenol-etc-6-d-for-five-minutes-70-to-80-c-the-browniiifj-of-media-due-to-excess-of-sodiuiu-nitrite-must-not-be-mistaken-for-this-pink-or-red-reaction-uninoculated-tubes-should-be-inchided-in-the-test-wliich-may-be-made-on-the-second-and-tenth-day-for-methods-of-determining-phenol-see-lewandowski-in-deutsche-med-wochenschrift-1890-p-1186-and-chesters-manual-p-t-schmidt-bd-ii-p-1008-gives-the-following-as-a-qualitative-reaction-for-tyrosiu-dissolve-!gt-boiling-in-water-and-add-a-s-image232254303.htmlRMRDT2MF–. Bacteria in relation to plant diseases. Bacteriology; Plant diseases. INDOL, PHENOL, ETC. 6:. d for five minutes (70° to 80° C). The browniiifj of media due to excess of sodiuiu nitrite must not be mistaken for this pink or red reaction. Uninoculated tubes should be inchided in the test, wliich may be made on the second and tenth day. For methods of determining phenol see Lewandowski in Deutsche Med. Wochenschrift, 1890, p. 1186, and Chester's Manual, p. t,;},. Schmidt (Bd. II, p. 1008) gives the following as a qualitative reaction for tyrosiu : Dissolve !:>}' boiling in water and add a s
. A manual of bacteriology for agricultural and general science students. Bacteriology. BACTEEIA OF THE AIE 49 Two students working together should prepare the following simple apparatus: Place 100 cc. of physiological salt solution (6 g. XaCl in 1000 cc. water) in a 500-cc. Erlenmeyer flask. Close the flask with a tight-fitting rubber stopper through which two bent glass tubes are passed. One of the tubes ends about 2 mm. from the bottom of the flask. The ends of the tubes are plugged with cotton and the flask is sterilized in the autoclave. Place 5 liters of water in a large bottle and mark Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-manual-of-bacteriology-for-agricultural-and-general-science-students-bacteriology-bacteeia-of-the-aie-49-two-students-working-together-should-prepare-the-following-simple-apparatus-place-100-cc-of-physiological-salt-solution-6-g-xacl-in-1000-cc-water-in-a-500-cc-erlenmeyer-flask-close-the-flask-with-a-tight-fitting-rubber-stopper-through-which-two-bent-glass-tubes-are-passed-one-of-the-tubes-ends-about-2-mm-from-the-bottom-of-the-flask-the-ends-of-the-tubes-are-plugged-with-cotton-and-the-flask-is-sterilized-in-the-autoclave-place-5-liters-of-water-in-a-large-bottle-and-mark-image232444508.htmlRMRE4N9G–. A manual of bacteriology for agricultural and general science students. Bacteriology. BACTEEIA OF THE AIE 49 Two students working together should prepare the following simple apparatus: Place 100 cc. of physiological salt solution (6 g. XaCl in 1000 cc. water) in a 500-cc. Erlenmeyer flask. Close the flask with a tight-fitting rubber stopper through which two bent glass tubes are passed. One of the tubes ends about 2 mm. from the bottom of the flask. The ends of the tubes are plugged with cotton and the flask is sterilized in the autoclave. Place 5 liters of water in a large bottle and mark
. Bacteriology in medicine and surgery. A practical manual for physicians, health officers, and students. Bacteriology. BACTEBIOLOGWAL TECHNIQUE. 221 ent media are stored in large quantities in round or flat- bottomed Erlenmeyer flasks (Fig. 23). From these, as needed, glass tubes (Fig. 24) are filled. Glass dishes with covers (Petri dishes. Fig. 27) and flat flasks are used for growing bacteria in or upon thin layers of media. When small amounts of media are taken fre- quently from flasks, Pasteur's flasks (Fig. 25) are of Fig. 24. Fig. 23.. Erlenmeyer flask. Fig. 25. Pasteur flask. Glass tes Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriology-in-medicine-and-surgery-a-practical-manual-for-physicians-health-officers-and-students-bacteriology-bactebiologwal-technique-221-ent-media-are-stored-in-large-quantities-in-round-or-flat-bottomed-erlenmeyer-flasks-fig-23-from-these-as-needed-glass-tubes-fig-24-are-filled-glass-dishes-with-covers-petri-dishes-fig-27-and-flat-flasks-are-used-for-growing-bacteria-in-or-upon-thin-layers-of-media-when-small-amounts-of-media-are-taken-fre-quently-from-flasks-pasteurs-flasks-fig-25-are-of-fig-24-fig-23-erlenmeyer-flask-fig-25-pasteur-flask-glass-tes-image232433966.htmlRMRE47W2–. Bacteriology in medicine and surgery. A practical manual for physicians, health officers, and students. Bacteriology. BACTEBIOLOGWAL TECHNIQUE. 221 ent media are stored in large quantities in round or flat- bottomed Erlenmeyer flasks (Fig. 23). From these, as needed, glass tubes (Fig. 24) are filled. Glass dishes with covers (Petri dishes. Fig. 27) and flat flasks are used for growing bacteria in or upon thin layers of media. When small amounts of media are taken fre- quently from flasks, Pasteur's flasks (Fig. 25) are of Fig. 24. Fig. 23.. Erlenmeyer flask. Fig. 25. Pasteur flask. Glass tes
. A manual of bacteriology for agricultural and general science students. Bacteriology. APPENDIX A 135 U tubes, A and A', filled with, sulphuric acid, are used to purify the air. £ is a long, hard-glass tube enlarged at one end for sterile cot- ton and stopper. A and B are for use in the aeration of the soil (if liquid culture media are used, these may be omitted). Tubes D, E, and A serve for watering; the sterile air is passed through A, and E then carries the water in flask 2 over into culture vessel 1. The large opening, C, in flask 1 carries a cylinder constructed as shown by Fig. 45. A wi Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-manual-of-bacteriology-for-agricultural-and-general-science-students-bacteriology-appendix-a-135-u-tubes-a-and-a-filled-with-sulphuric-acid-are-used-to-purify-the-air-is-a-long-hard-glass-tube-enlarged-at-one-end-for-sterile-cot-ton-and-stopper-a-and-b-are-for-use-in-the-aeration-of-the-soil-if-liquid-culture-media-are-used-these-may-be-omitted-tubes-d-e-and-a-serve-for-watering-the-sterile-air-is-passed-through-a-and-e-then-carries-the-water-in-flask-2-over-into-culture-vessel-1-the-large-opening-c-in-flask-1-carries-a-cylinder-constructed-as-shown-by-fig-45-a-wi-image232444433.htmlRMRE4N6W–. A manual of bacteriology for agricultural and general science students. Bacteriology. APPENDIX A 135 U tubes, A and A', filled with, sulphuric acid, are used to purify the air. £ is a long, hard-glass tube enlarged at one end for sterile cot- ton and stopper. A and B are for use in the aeration of the soil (if liquid culture media are used, these may be omitted). Tubes D, E, and A serve for watering; the sterile air is passed through A, and E then carries the water in flask 2 over into culture vessel 1. The large opening, C, in flask 1 carries a cylinder constructed as shown by Fig. 45. A wi
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 172 Sterilization and Disinfection and the vacuum be slowly relieved. If the valve be opened suddenly the fluids boil rapidly and the cotton plugs maybe forced into the tubes or flasks by the air pressure. The chief objection to the use of the autoclave is that the high temperature sometimes brings about chemic changes in the media by which the reaction is altered. Sterilization by Filtration.—Liquids that cannot be subjected to heat without the loss of Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-upon-the-pathogenic-bacteria-and-protozoa-for-students-of-medicine-and-physicians-bacteriology-pathogenic-bacteria-protozoa-172-sterilization-and-disinfection-and-the-vacuum-be-slowly-relieved-if-the-valve-be-opened-suddenly-the-fluids-boil-rapidly-and-the-cotton-plugs-maybe-forced-into-the-tubes-or-flasks-by-the-air-pressure-the-chief-objection-to-the-use-of-the-autoclave-is-that-the-high-temperature-sometimes-brings-about-chemic-changes-in-the-media-by-which-the-reaction-is-altered-sterilization-by-filtrationliquids-that-cannot-be-subjected-to-heat-without-the-loss-of-image232442240.htmlRMRE4JCG–. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 172 Sterilization and Disinfection and the vacuum be slowly relieved. If the valve be opened suddenly the fluids boil rapidly and the cotton plugs maybe forced into the tubes or flasks by the air pressure. The chief objection to the use of the autoclave is that the high temperature sometimes brings about chemic changes in the media by which the reaction is altered. Sterilization by Filtration.—Liquids that cannot be subjected to heat without the loss of
. Bacteriology and surgical technics for nurses. Surgical nursing; Operations, Surgical; Bacteriology. l6o SURGICAL TECHNIC SPECIAL METHODS OF ANESTHESIA Intrapharyngeal Inhalation Anesthesia.—In operations about the mouth, face, etc., this method, by which tubes are passed into the pharynx through the nose (Crile's method), will be found to be very convenient. The advantages are that the anesthetizer and his apparatus. Fig. 138.—Showing the method of inserting the tubes and packing the pharynx for intubation anesthesia. (Morrow's Diagnostic and Thera- peutic Technic.) are removed from the fie Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriology-and-surgical-technics-for-nurses-surgical-nursing-operations-surgical-bacteriology-l6o-surgical-technic-special-methods-of-anesthesia-intrapharyngeal-inhalation-anesthesiain-operations-about-the-mouth-face-etc-this-method-by-which-tubes-are-passed-into-the-pharynx-through-the-nose-criles-method-will-be-found-to-be-very-convenient-the-advantages-are-that-the-anesthetizer-and-his-apparatus-fig-138showing-the-method-of-inserting-the-tubes-and-packing-the-pharynx-for-intubation-anesthesia-morrows-diagnostic-and-thera-peutic-technic-are-removed-from-the-fie-image235268013.htmlRMRJNAN1–. Bacteriology and surgical technics for nurses. Surgical nursing; Operations, Surgical; Bacteriology. l6o SURGICAL TECHNIC SPECIAL METHODS OF ANESTHESIA Intrapharyngeal Inhalation Anesthesia.—In operations about the mouth, face, etc., this method, by which tubes are passed into the pharynx through the nose (Crile's method), will be found to be very convenient. The advantages are that the anesthetizer and his apparatus. Fig. 138.—Showing the method of inserting the tubes and packing the pharynx for intubation anesthesia. (Morrow's Diagnostic and Thera- peutic Technic.) are removed from the fie
. Manual of bacteriology. Bacteriology. KOCH'S METHOD OF PREPARING PLATES. 55 through the bottom. From their characters, colour, shape, contour, appearance of surface, liquefaction or non-liquefaction of the gelatin, etc., the colonies can be classified into groups. Further aid in the grouping of the varieties is obtained by making film preparations and examining them microscopically. Gelatin or agar tubes may then be inoculated from a colony of each variety, and the growths obtained are then examined both as to their purity and as to their special characters, with a view to their identificati Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/manual-of-bacteriology-bacteriology-kochs-method-of-preparing-plates-55-through-the-bottom-from-their-characters-colour-shape-contour-appearance-of-surface-liquefaction-or-non-liquefaction-of-the-gelatin-etc-the-colonies-can-be-classified-into-groups-further-aid-in-the-grouping-of-the-varieties-is-obtained-by-making-film-preparations-and-examining-them-microscopically-gelatin-or-agar-tubes-may-then-be-inoculated-from-a-colony-of-each-variety-and-the-growths-obtained-are-then-examined-both-as-to-their-purity-and-as-to-their-special-characters-with-a-view-to-their-identificati-image232443918.htmlRMRE4MGE–. Manual of bacteriology. Bacteriology. KOCH'S METHOD OF PREPARING PLATES. 55 through the bottom. From their characters, colour, shape, contour, appearance of surface, liquefaction or non-liquefaction of the gelatin, etc., the colonies can be classified into groups. Further aid in the grouping of the varieties is obtained by making film preparations and examining them microscopically. Gelatin or agar tubes may then be inoculated from a colony of each variety, and the growths obtained are then examined both as to their purity and as to their special characters, with a view to their identificati
. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. Fig. 37.—Graduated fermentation tube. These tubes are required for gas determination with colon bacillus and other gas-forming micro-organisms. aerobic or essentially anaerobic; the manner of determining the thermal death- point; relationship of rate of growth to temperature, etc. We have said nothing of the use of indicators added to culture media, as litmus, rosolic acid, and phenolphthalein, nor have we explained the special use of special culture media in determining the nature and identi Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pharmaceutical-bacteriology-with-special-reference-to-disinfection-and-sterilization-bacteriology-fig-37graduated-fermentation-tube-these-tubes-are-required-for-gas-determination-with-colon-bacillus-and-other-gas-forming-micro-organisms-aerobic-or-essentially-anaerobic-the-manner-of-determining-the-thermal-death-point-relationship-of-rate-of-growth-to-temperature-etc-we-have-said-nothing-of-the-use-of-indicators-added-to-culture-media-as-litmus-rosolic-acid-and-phenolphthalein-nor-have-we-explained-the-special-use-of-special-culture-media-in-determining-the-nature-and-identi-image232385929.htmlRMRE22HD–. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. Fig. 37.—Graduated fermentation tube. These tubes are required for gas determination with colon bacillus and other gas-forming micro-organisms. aerobic or essentially anaerobic; the manner of determining the thermal death- point; relationship of rate of growth to temperature, etc. We have said nothing of the use of indicators added to culture media, as litmus, rosolic acid, and phenolphthalein, nor have we explained the special use of special culture media in determining the nature and identi
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. The Hemolytic Amboceptor 293 stroma dissolves, sometimes it sediments as a colorless mass to the bottom of the tube. In the tubes containing the positive or syphilitic serum, and in which there is complete complement fixation, the unaltered cor- puscles sediment to the bottom of the tube, leaving a colorless fluid above. When the complement fixation is complete there is no solution of the hemoglobin. Such a result has been described by Citron as + -|- + Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-upon-the-pathogenic-bacteria-and-protozoa-for-students-of-medicine-and-physicians-bacteriology-pathogenic-bacteria-protozoa-the-hemolytic-amboceptor-293-stroma-dissolves-sometimes-it-sediments-as-a-colorless-mass-to-the-bottom-of-the-tube-in-the-tubes-containing-the-positive-or-syphilitic-serum-and-in-which-there-is-complete-complement-fixation-the-unaltered-cor-puscles-sediment-to-the-bottom-of-the-tube-leaving-a-colorless-fluid-above-when-the-complement-fixation-is-complete-there-is-no-solution-of-the-hemoglobin-such-a-result-has-been-described-by-citron-as-image232441374.htmlRMRE4H9J–. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. The Hemolytic Amboceptor 293 stroma dissolves, sometimes it sediments as a colorless mass to the bottom of the tube. In the tubes containing the positive or syphilitic serum, and in which there is complete complement fixation, the unaltered cor- puscles sediment to the bottom of the tube, leaving a colorless fluid above. When the complement fixation is complete there is no solution of the hemoglobin. Such a result has been described by Citron as + -|- +
. A manual of bacteriology for agricultural and general science students. Bacteriology. Fig. 5. Method for filling test tubes with liquid media. (After Russell and Hastings) Hold the tip free from the walls of the tube and avoid smearing the mouth of the tube with the medium. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Reed, Howard Sprague, 1876-. Boston, New York, Ginn Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-manual-of-bacteriology-for-agricultural-and-general-science-students-bacteriology-fig-5-method-for-filling-test-tubes-with-liquid-media-after-russell-and-hastings-hold-the-tip-free-from-the-walls-of-the-tube-and-avoid-smearing-the-mouth-of-the-tube-with-the-medium-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-digitally-enhanced-for-readability-coloration-and-appearance-of-these-illustrations-may-not-perfectly-resemble-the-original-work-reed-howard-sprague-1876-boston-new-york-ginn-image232435207.htmlRMRE49DB–. A manual of bacteriology for agricultural and general science students. Bacteriology. Fig. 5. Method for filling test tubes with liquid media. (After Russell and Hastings) Hold the tip free from the walls of the tube and avoid smearing the mouth of the tube with the medium. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Reed, Howard Sprague, 1876-. Boston, New York, Ginn
![. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. ]14 BACTERIOLOGY. plugged sterilised test-tubes. These should be filled, with a sterilised pipette, to about one-third of their capacity. Formerly the tubes were sterilised by Tyndall's process of dis- continuous sterilisation. The tubes were placed in Koch's serum sterilisei-, with the temperature maintained for an hour or more at .56° C, and this was repeated for six successive days, the temperature on the last day be Stock Photo](https://c8.alamy.com/comp/RE4HJ9/a-text-book-of-bacteriology-including-the-etiology-and-prevention-of-infective-diseases-and-a-short-account-of-yeasts-and-moulds-haematazoa-and-psorosperms-bacteriology-14-bacteriology-plugged-sterilised-test-tubes-these-should-be-filled-with-a-sterilised-pipette-to-about-one-third-of-their-capacity-formerly-the-tubes-were-sterilised-by-tyndalls-process-of-dis-continuous-sterilisation-the-tubes-were-placed-in-kochs-serum-sterilisei-with-the-temperature-maintained-for-an-hour-or-more-at-56-c-and-this-was-repeated-for-six-successive-days-the-temperature-on-the-last-day-be-RE4HJ9.jpg ". A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. ]14 BACTERIOLOGY. plugged sterilised test-tubes. These should be filled, with a sterilised pipette, to about one-third of their capacity. Formerly the tubes were sterilised by Tyndall's process of dis- continuous sterilisation. The tubes were placed in Koch's serum sterilisei-, with the temperature maintained for an hour or more at .56° C, and this was repeated for six successive days, the temperature on the last day be Stock Photo")
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. ]14 BACTERIOLOGY. plugged sterilised test-tubes. These should be filled, with a sterilised pipette, to about one-third of their capacity. Formerly the tubes were sterilised by Tyndall's process of dis- continuous sterilisation. The tubes were placed in Koch's serum sterilisei-, with the temperature maintained for an hour or more at .56° C, and this was repeated for six successive days, the temperature on the last day be Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-bacteriology-including-the-etiology-and-prevention-of-infective-diseases-and-a-short-account-of-yeasts-and-moulds-haematazoa-and-psorosperms-bacteriology-14-bacteriology-plugged-sterilised-test-tubes-these-should-be-filled-with-a-sterilised-pipette-to-about-one-third-of-their-capacity-formerly-the-tubes-were-sterilised-by-tyndalls-process-of-dis-continuous-sterilisation-the-tubes-were-placed-in-kochs-serum-sterilisei-with-the-temperature-maintained-for-an-hour-or-more-at-56-c-and-this-was-repeated-for-six-successive-days-the-temperature-on-the-last-day-be-image232441617.htmlRMRE4HJ9–. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. ]14 BACTERIOLOGY. plugged sterilised test-tubes. These should be filled, with a sterilised pipette, to about one-third of their capacity. Formerly the tubes were sterilised by Tyndall's process of dis- continuous sterilisation. The tubes were placed in Koch's serum sterilisei-, with the temperature maintained for an hour or more at .56° C, and this was repeated for six successive days, the temperature on the last day be
. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. YEASTS AND MOULDS. 145 believed to be pathogenic in chronic catarrhal conditions of the Eustachian tubes and of the stomach. b. Penicillium glaucum. This is the omnipresent blue-green mould so common in the household, infesting all exposed moist organic substances. Supposed to be non-pathogenic, although some credit it with being the cause of pellagra. c. Aspergillus fumigatus. Said to be the cause of pellagra. d. Aspergillus concentricits. Causes ringworm. Common in the. Fig. 66.—Saccharomyc Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pharmaceutical-bacteriology-with-special-reference-to-disinfection-and-sterilization-bacteriology-yeasts-and-moulds-145-believed-to-be-pathogenic-in-chronic-catarrhal-conditions-of-the-eustachian-tubes-and-of-the-stomach-b-penicillium-glaucum-this-is-the-omnipresent-blue-green-mould-so-common-in-the-household-infesting-all-exposed-moist-organic-substances-supposed-to-be-non-pathogenic-although-some-credit-it-with-being-the-cause-of-pellagra-c-aspergillus-fumigatus-said-to-be-the-cause-of-pellagra-d-aspergillus-concentricits-causes-ringworm-common-in-the-fig-66saccharomyc-image232385794.htmlRMRE22CJ–. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. YEASTS AND MOULDS. 145 believed to be pathogenic in chronic catarrhal conditions of the Eustachian tubes and of the stomach. b. Penicillium glaucum. This is the omnipresent blue-green mould so common in the household, infesting all exposed moist organic substances. Supposed to be non-pathogenic, although some credit it with being the cause of pellagra. c. Aspergillus fumigatus. Said to be the cause of pellagra. d. Aspergillus concentricits. Causes ringworm. Common in the. Fig. 66.—Saccharomyc
. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. S6 PHARMACEUTICAL BACTERIOLOGY. h. Deep stab culture—where is growth most active? If at bottom, it indicates anaerobic tendencies. If limited to top of medium, it indicates de- cidely aerobic tendencies. (Most bacteria are decidedly,aerobic; that is, they require oxygen to thrive.) The test-tube cultures do not necessarily represent pure cultures, and the student cannot know whether the growths in the test-tubes represent the predominating bacterial flora in the substance from which the inocu Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pharmaceutical-bacteriology-with-special-reference-to-disinfection-and-sterilization-bacteriology-s6-pharmaceutical-bacteriology-h-deep-stab-culturewhere-is-growth-most-active-if-at-bottom-it-indicates-anaerobic-tendencies-if-limited-to-top-of-medium-it-indicates-de-cidely-aerobic-tendencies-most-bacteria-are-decidedlyaerobic-that-is-they-require-oxygen-to-thrive-the-test-tube-cultures-do-not-necessarily-represent-pure-cultures-and-the-student-cannot-know-whether-the-growths-in-the-test-tubes-represent-the-predominating-bacterial-flora-in-the-substance-from-which-the-inocu-image232385945.htmlRMRE22J1–. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. S6 PHARMACEUTICAL BACTERIOLOGY. h. Deep stab culture—where is growth most active? If at bottom, it indicates anaerobic tendencies. If limited to top of medium, it indicates de- cidely aerobic tendencies. (Most bacteria are decidedly,aerobic; that is, they require oxygen to thrive.) The test-tube cultures do not necessarily represent pure cultures, and the student cannot know whether the growths in the test-tubes represent the predominating bacterial flora in the substance from which the inocu
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Technic of Culture Manipulation 20S biers, racks, or other contrivances, and not lay them upon the table so that the contents touch the stoppers. When the cotton plugs are removed in order that the contents of the tubes or flasks may be inoculated or otherwise manipulated, the removal and replacement should be done as quickly as convenient, and the mouth of the tube should be flamed before removal. The plugs should be held between the fingers, by that pa Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-upon-the-pathogenic-bacteria-and-protozoa-for-students-of-medicine-and-physicians-bacteriology-pathogenic-bacteria-protozoa-technic-of-culture-manipulation-20s-biers-racks-or-other-contrivances-and-not-lay-them-upon-the-table-so-that-the-contents-touch-the-stoppers-when-the-cotton-plugs-are-removed-in-order-that-the-contents-of-the-tubes-or-flasks-may-be-inoculated-or-otherwise-manipulated-the-removal-and-replacement-should-be-done-as-quickly-as-convenient-and-the-mouth-of-the-tube-should-be-flamed-before-removal-the-plugs-should-be-held-between-the-fingers-by-that-pa-image232353815.htmlRMRE0HJF–. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Technic of Culture Manipulation 20S biers, racks, or other contrivances, and not lay them upon the table so that the contents touch the stoppers. When the cotton plugs are removed in order that the contents of the tubes or flasks may be inoculated or otherwise manipulated, the removal and replacement should be done as quickly as convenient, and the mouth of the tube should be flamed before removal. The plugs should be held between the fingers, by that pa
. Bacteriological apparatus : pathological, biochemical. Scientific apparatus and instruments; Bacteriology; Chemical industry. 17831 17831 Supports—Test Tube. For drying test tubes. No. A B No. of pins 25 50 Each 2.00 2.25. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Will Corporation. Rochester, N. Y. : The Will Corporation Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-apparatus-pathological-biochemical-scientific-apparatus-and-instruments-bacteriology-chemical-industry-17831-17831-supportstest-tube-for-drying-test-tubes-no-a-b-no-of-pins-25-50-each-200-225-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-digitally-enhanced-for-readability-coloration-and-appearance-of-these-illustrations-may-not-perfectly-resemble-the-original-work-will-corporation-rochester-n-y-the-will-corporation-image235258268.htmlRMRJMX90–. Bacteriological apparatus : pathological, biochemical. Scientific apparatus and instruments; Bacteriology; Chemical industry. 17831 17831 Supports—Test Tube. For drying test tubes. No. A B No. of pins 25 50 Each 2.00 2.25. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Will Corporation. Rochester, N. Y. : The Will Corporation
. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration; Bacteriology. 112 BACTERIOLOGICAL METHODS The tubes must be fastened to suitable stands or supports to pre- vent, as much as possible, the mechanical mixing of the contents after the inoculations are made. It is perhaps self-evident that concentrates or high contaminations are to be inoculated into the tubes. The tubes should be large enough to hold at least 50 to 100 cc. of medium and suspected water in equal parts. 12. Possible Contamination of Foo Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-methods-in-food-and-drug-laboratories-with-an-introduction-to-micro-analytical-methods-bacteriology-food-drug-adulteration-bacteriology-112-bacteriological-methods-the-tubes-must-be-fastened-to-suitable-stands-or-supports-to-pre-vent-as-much-as-possible-the-mechanical-mixing-of-the-contents-after-the-inoculations-are-made-it-is-perhaps-self-evident-that-concentrates-or-high-contaminations-are-to-be-inoculated-into-the-tubes-the-tubes-should-be-large-enough-to-hold-at-least-50-to-100-cc-of-medium-and-suspected-water-in-equal-parts-12-possible-contamination-of-foo-image235251085.htmlRMRJMH4D–. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration; Bacteriology. 112 BACTERIOLOGICAL METHODS The tubes must be fastened to suitable stands or supports to pre- vent, as much as possible, the mechanical mixing of the contents after the inoculations are made. It is perhaps self-evident that concentrates or high contaminations are to be inoculated into the tubes. The tubes should be large enough to hold at least 50 to 100 cc. of medium and suspected water in equal parts. 12. Possible Contamination of Foo
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. APPARATUS, MATERIAL, AND REAGENTS. 631 Bulbed Tubes.—Glass vessels, such as test-tubes, flasks and pipettes, which are used in dealing with liquid media, have already been mentioned under other headings; but bulbed tubes, Pasteur's bulbs, and various other forms are also required for special experiments. (L) Apparatus for Incubation. There are several forms of incubator, each of which has its advocates. They are mostly Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-bacteriology-including-the-etiology-and-prevention-of-infective-diseases-and-a-short-account-of-yeasts-and-moulds-haematazoa-and-psorosperms-bacteriology-apparatus-material-and-reagents-631-bulbed-tubesglass-vessels-such-as-test-tubes-flasks-and-pipettes-which-are-used-in-dealing-with-liquid-media-have-already-been-mentioned-under-other-headings-but-bulbed-tubes-pasteurs-bulbs-and-various-other-forms-are-also-required-for-special-experiments-l-apparatus-for-incubation-there-are-several-forms-of-incubator-each-of-which-has-its-advocates-they-are-mostly-image232383739.htmlRMRE1YR7–. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. APPARATUS, MATERIAL, AND REAGENTS. 631 Bulbed Tubes.—Glass vessels, such as test-tubes, flasks and pipettes, which are used in dealing with liquid media, have already been mentioned under other headings; but bulbed tubes, Pasteur's bulbs, and various other forms are also required for special experiments. (L) Apparatus for Incubation. There are several forms of incubator, each of which has its advocates. They are mostly
. Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians. Bacteriology. 70 APPLIED BACTEEIOLOGY soon as they are quite solidified they are set aside for the colonies to develop. These tubes possess the advantage that they are quickly made, they do not occupy much room, and are well protected against atmospheric germs.. Fig. 16.—Method of making Esmaroh's Eoll Cuiturb. When the colonies have formed, they can readily be counted and examined by means of a lens. Agar Plates.—The characters of the growth on nutrient agar media ar Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/applied-bacteriology-an-introductory-handbook-for-the-use-of-students-medical-officers-of-health-analysts-and-sanitarians-bacteriology-70-applied-bacteeiology-soon-as-they-are-quite-solidified-they-are-set-aside-for-the-colonies-to-develop-these-tubes-possess-the-advantage-that-they-are-quickly-made-they-do-not-occupy-much-room-and-are-well-protected-against-atmospheric-germs-fig-16method-of-making-esmarohs-eoll-cuiturb-when-the-colonies-have-formed-they-can-readily-be-counted-and-examined-by-means-of-a-lens-agar-platesthe-characters-of-the-growth-on-nutrient-agar-media-ar-image232434101.htmlRMRE481W–. Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians. Bacteriology. 70 APPLIED BACTEEIOLOGY soon as they are quite solidified they are set aside for the colonies to develop. These tubes possess the advantage that they are quickly made, they do not occupy much room, and are well protected against atmospheric germs.. Fig. 16.—Method of making Esmaroh's Eoll Cuiturb. When the colonies have formed, they can readily be counted and examined by means of a lens. Agar Plates.—The characters of the growth on nutrient agar media ar
. Bacteriological methods in food and drugs laboratories : with an introduction to micro-analytical methods . Bacteriology; Food; Drugs. 112 BACTERIOLOGICAL METHODS The tubes must be fastened to suitable stands or supports to pre- vent, as much as possible, the mechanical mixing of the contents after the inoculations are made. It is perhaps self-evident that concentrates or high contaminations are to be inoculated into the tubes. The tubes should be large enough to hold at least 50 to 100 cc. of medium and suspected water in equal parts. 12. Possible Contamination of Food Substances with the C Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-methods-in-food-and-drugs-laboratories-with-an-introduction-to-micro-analytical-methods-bacteriology-food-drugs-112-bacteriological-methods-the-tubes-must-be-fastened-to-suitable-stands-or-supports-to-pre-vent-as-much-as-possible-the-mechanical-mixing-of-the-contents-after-the-inoculations-are-made-it-is-perhaps-self-evident-that-concentrates-or-high-contaminations-are-to-be-inoculated-into-the-tubes-the-tubes-should-be-large-enough-to-hold-at-least-50-to-100-cc-of-medium-and-suspected-water-in-equal-parts-12-possible-contamination-of-food-substances-with-the-c-image232353318.htmlRMRE0H0P–. Bacteriological methods in food and drugs laboratories : with an introduction to micro-analytical methods . Bacteriology; Food; Drugs. 112 BACTERIOLOGICAL METHODS The tubes must be fastened to suitable stands or supports to pre- vent, as much as possible, the mechanical mixing of the contents after the inoculations are made. It is perhaps self-evident that concentrates or high contaminations are to be inoculated into the tubes. The tubes should be large enough to hold at least 50 to 100 cc. of medium and suspected water in equal parts. 12. Possible Contamination of Food Substances with the C
. Laboratory work in bacteriology. Bacteriology. 244 BACTEBIOLOGY. der to obtain results. The apparatus employed for this purpose is known as an incubator or thermostat. It is made of heavy copper and has double walls. The space between these, which should be as large as possible, is filled with distilled water. In form the thermostat may be oblong, square or oval. Pig. 36 shows an excellent thermostat of oval form.. Fig. 36. The incubator or thermostat. It is desirable that the incubator should have as much available space as possible for dishes, flasks, tubes, etc. The apparatus as ordinaril Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/laboratory-work-in-bacteriology-bacteriology-244-bactebiology-der-to-obtain-results-the-apparatus-employed-for-this-purpose-is-known-as-an-incubator-or-thermostat-it-is-made-of-heavy-copper-and-has-double-walls-the-space-between-these-which-should-be-as-large-as-possible-is-filled-with-distilled-water-in-form-the-thermostat-may-be-oblong-square-or-oval-pig-36-shows-an-excellent-thermostat-of-oval-form-fig-36-the-incubator-or-thermostat-it-is-desirable-that-the-incubator-should-have-as-much-available-space-as-possible-for-dishes-flasks-tubes-etc-the-apparatus-as-ordinaril-image232077119.htmlRMRDG0MF–. Laboratory work in bacteriology. Bacteriology. 244 BACTEBIOLOGY. der to obtain results. The apparatus employed for this purpose is known as an incubator or thermostat. It is made of heavy copper and has double walls. The space between these, which should be as large as possible, is filled with distilled water. In form the thermostat may be oblong, square or oval. Pig. 36 shows an excellent thermostat of oval form.. Fig. 36. The incubator or thermostat. It is desirable that the incubator should have as much available space as possible for dishes, flasks, tubes, etc. The apparatus as ordinaril
. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration. 112 BACTERIOLOGICAL METHODS The tubes must be fastened to suitable stands or supports to pre- vent, as much as possible, the mechanical mixing of the contents after the inoculations are made. It is perhaps self-evident that concentrates or high contaminations are to be inoculated into the tubes. The tubes should be large enough to hold at least 50 to 100 cc. of medium and suspected water in equal parts. 12. Possible Contamination of Food Substances w Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-methods-in-food-and-drug-laboratories-with-an-introduction-to-micro-analytical-methods-bacteriology-food-drug-adulteration-112-bacteriological-methods-the-tubes-must-be-fastened-to-suitable-stands-or-supports-to-pre-vent-as-much-as-possible-the-mechanical-mixing-of-the-contents-after-the-inoculations-are-made-it-is-perhaps-self-evident-that-concentrates-or-high-contaminations-are-to-be-inoculated-into-the-tubes-the-tubes-should-be-large-enough-to-hold-at-least-50-to-100-cc-of-medium-and-suspected-water-in-equal-parts-12-possible-contamination-of-food-substances-w-image235271075.htmlRMRJNEJB–. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration. 112 BACTERIOLOGICAL METHODS The tubes must be fastened to suitable stands or supports to pre- vent, as much as possible, the mechanical mixing of the contents after the inoculations are made. It is perhaps self-evident that concentrates or high contaminations are to be inoculated into the tubes. The tubes should be large enough to hold at least 50 to 100 cc. of medium and suspected water in equal parts. 12. Possible Contamination of Food Substances w
. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. 112 BACTERIOLOGY of the wire from the first tube to the second. When the needle is introduced into or removed from either tube it should not touch the side of the tube at any point,and should only come in contact with the region desired. After inoculation of the second tube has been effected, the wire is heated to redness in the flame, the necks of the tubes are passed through the flame, and the plugs are returned to their re Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathogenic-micro-organisms-a-text-book-of-microbiology-for-physicians-and-students-of-medicine-based-upon-williams-bacteriology-bacteriology-pathogenic-bacteria-112-bacteriology-of-the-wire-from-the-first-tube-to-the-second-when-the-needle-is-introduced-into-or-removed-from-either-tube-it-should-not-touch-the-side-of-the-tube-at-any-pointand-should-only-come-in-contact-with-the-region-desired-after-inoculation-of-the-second-tube-has-been-effected-the-wire-is-heated-to-redness-in-the-flame-the-necks-of-the-tubes-are-passed-through-the-flame-and-the-plugs-are-returned-to-their-re-image232419432.htmlRMRE3HA0–. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. 112 BACTERIOLOGY of the wire from the first tube to the second. When the needle is introduced into or removed from either tube it should not touch the side of the tube at any point,and should only come in contact with the region desired. After inoculation of the second tube has been effected, the wire is heated to redness in the flame, the necks of the tubes are passed through the flame, and the plugs are returned to their re
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Technic of Culture Manipulation 203 biers, racks, or otlier contrivances, and not lay tliem upon the table so that the contents touch the stoppers. When the cotton plugs are removed in order that the contents of the tubes or flasks may be inoculated or otherwise manipulated, the removal and replacement should be done as quickly as convenient, and the mouth of the tube should be flamed before removal. The plugs should be held between the fingers, by that Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-upon-the-pathogenic-bacteria-and-protozoa-for-students-of-medicine-and-physicians-bacteriology-pathogenic-bacteria-protozoa-technic-of-culture-manipulation-203-biers-racks-or-otlier-contrivances-and-not-lay-tliem-upon-the-table-so-that-the-contents-touch-the-stoppers-when-the-cotton-plugs-are-removed-in-order-that-the-contents-of-the-tubes-or-flasks-may-be-inoculated-or-otherwise-manipulated-the-removal-and-replacement-should-be-done-as-quickly-as-convenient-and-the-mouth-of-the-tube-should-be-flamed-before-removal-the-plugs-should-be-held-between-the-fingers-by-that-image232442169.htmlRMRE4JA1–. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Technic of Culture Manipulation 203 biers, racks, or otlier contrivances, and not lay tliem upon the table so that the contents touch the stoppers. When the cotton plugs are removed in order that the contents of the tubes or flasks may be inoculated or otherwise manipulated, the removal and replacement should be done as quickly as convenient, and the mouth of the tube should be flamed before removal. The plugs should be held between the fingers, by that
. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. THE CULTIVATION OF MICRO-ORGANISMS III portion of the material to be examined—pus, for example—and introduce it with a sterilized platinum wire or loop into one of the tubes. The plug of the test-tube is to be withdrawn, twisting it slightly, taking it between the third and fourth fingers of the left hand, with the part that projects into the tube pointing to- ward the back of the hand. It must not be allowed to touch any obj Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/pathogenic-micro-organisms-a-text-book-of-microbiology-for-physicians-and-students-of-medicine-based-upon-williams-bacteriology-bacteriology-pathogenic-bacteria-the-cultivation-of-micro-organisms-iii-portion-of-the-material-to-be-examinedpus-for-exampleand-introduce-it-with-a-sterilized-platinum-wire-or-loop-into-one-of-the-tubes-the-plug-of-the-test-tube-is-to-be-withdrawn-twisting-it-slightly-taking-it-between-the-third-and-fourth-fingers-of-the-left-hand-with-the-part-that-projects-into-the-tube-pointing-to-ward-the-back-of-the-hand-it-must-not-be-allowed-to-touch-any-obj-image232419435.htmlRMRE3HA3–. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. THE CULTIVATION OF MICRO-ORGANISMS III portion of the material to be examined—pus, for example—and introduce it with a sterilized platinum wire or loop into one of the tubes. The plug of the test-tube is to be withdrawn, twisting it slightly, taking it between the third and fourth fingers of the left hand, with the part that projects into the tube pointing to- ward the back of the hand. It must not be allowed to touch any obj
. The principles of bacteriology: a practical manual for students and physicians. Bacteriology. 190 BACTERIOLOGY. 32), the stem of which is bent to an angle of about 110° with the long axis of the body. The larger part of the aerobioscope is divided into squares to facilitate the counting of the colonies. By the employment of this apparatus one can make these analyses at any place, and can, without fear of con- tamination, carry the tubes to the laboratory, where the cultivation part of the work may be done. Fis. 32.. Aside from this advantage, the use of a vacuum- cylinder permits a known vol Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/the-principles-of-bacteriology-a-practical-manual-for-students-and-physicians-bacteriology-190-bacteriology-32-the-stem-of-which-is-bent-to-an-angle-of-about-110-with-the-long-axis-of-the-body-the-larger-part-of-the-aerobioscope-is-divided-into-squares-to-facilitate-the-counting-of-the-colonies-by-the-employment-of-this-apparatus-one-can-make-these-analyses-at-any-place-and-can-without-fear-of-con-tamination-carry-the-tubes-to-the-laboratory-where-the-cultivation-part-of-the-work-may-be-done-fis-32-aside-from-this-advantage-the-use-of-a-vacuum-cylinder-permits-a-known-vol-image232433310.htmlRMRE471J–. The principles of bacteriology: a practical manual for students and physicians. Bacteriology. 190 BACTERIOLOGY. 32), the stem of which is bent to an angle of about 110° with the long axis of the body. The larger part of the aerobioscope is divided into squares to facilitate the counting of the colonies. By the employment of this apparatus one can make these analyses at any place, and can, without fear of con- tamination, carry the tubes to the laboratory, where the cultivation part of the work may be done. Fis. 32.. Aside from this advantage, the use of a vacuum- cylinder permits a known vol
. Handbook of bacteriological diagnosis for practitioners, including instructions for the clinical examination of the blood. Bacteriology; Diagnosis. 156 BACTERIOLOGICAL DIAGNOSIS. degree of dilution. The other is graduated into a hundred parts, the graduation being such that when 20 cubic millimetres of normal blood are diluted with water up to the loo mark the colour of the two tubes should be exactly the same. A pipette measuring 20 cm. and a dropping bottle (which is to be filled with water) are also provided. Method of use.—Place a few drops of water (preferably, but not necessarily, dist Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/handbook-of-bacteriological-diagnosis-for-practitioners-including-instructions-for-the-clinical-examination-of-the-blood-bacteriology-diagnosis-156-bacteriological-diagnosis-degree-of-dilution-the-other-is-graduated-into-a-hundred-parts-the-graduation-being-such-that-when-20-cubic-millimetres-of-normal-blood-are-diluted-with-water-up-to-the-loo-mark-the-colour-of-the-two-tubes-should-be-exactly-the-same-a-pipette-measuring-20-cm-and-a-dropping-bottle-which-is-to-be-filled-with-water-are-also-provided-method-of-useplace-a-few-drops-of-water-preferably-but-not-necessarily-dist-image232115423.htmlRMRDHNGF–. Handbook of bacteriological diagnosis for practitioners, including instructions for the clinical examination of the blood. Bacteriology; Diagnosis. 156 BACTERIOLOGICAL DIAGNOSIS. degree of dilution. The other is graduated into a hundred parts, the graduation being such that when 20 cubic millimetres of normal blood are diluted with water up to the loo mark the colour of the two tubes should be exactly the same. A pipette measuring 20 cm. and a dropping bottle (which is to be filled with water) are also provided. Method of use.—Place a few drops of water (preferably, but not necessarily, dist
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. 'NUTRIENT MEDIA AND METHODS OF CULTIVATION. 115 Inoculation of the, Tubes.—A small portion of a culture or of the material to be inoculated is taken up with a sterilised platinum needle, and traced over the sloping surface of the serum; or a fragment of tissue, such as diphtheritic membrane or tubercle, may be introduced into the tube and rubbed gently over the serum so as not to Ijreak the sui'face. '. Fig. 44.—Hueppe' Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-bacteriology-including-the-etiology-and-prevention-of-infective-diseases-and-a-short-account-of-yeasts-and-moulds-haematazoa-and-psorosperms-bacteriology-nutrient-media-and-methods-of-cultivation-115-inoculation-of-the-tubesa-small-portion-of-a-culture-or-of-the-material-to-be-inoculated-is-taken-up-with-a-sterilised-platinum-needle-and-traced-over-the-sloping-surface-of-the-serum-or-a-fragment-of-tissue-such-as-diphtheritic-membrane-or-tubercle-may-be-introduced-into-the-tube-and-rubbed-gently-over-the-serum-so-as-not-to-ijreak-the-suiface-fig-44hueppe-image232441608.htmlRMRE4HJ0–. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. 'NUTRIENT MEDIA AND METHODS OF CULTIVATION. 115 Inoculation of the, Tubes.—A small portion of a culture or of the material to be inoculated is taken up with a sterilised platinum needle, and traced over the sloping surface of the serum; or a fragment of tissue, such as diphtheritic membrane or tubercle, may be introduced into the tube and rubbed gently over the serum so as not to Ijreak the sui'face. '. Fig. 44.—Hueppe'
. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration. STANDARDIZATION OF DISINFECTANTS 239 ning crosswise represent the same strength dilution, while the rows running lengthwise represent the different strengths to be used in the experiment. Starting with the lowest dilution (i.e., the strongest), the cylinder is shaken, then 5 cc. are measured into the tubes of the row to receive that strength, using a 5 cc. delivery pipette. In order to economize glassware, the same pipette is used for measuring out t Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-methods-in-food-and-drug-laboratories-with-an-introduction-to-micro-analytical-methods-bacteriology-food-drug-adulteration-standardization-of-disinfectants-239-ning-crosswise-represent-the-same-strength-dilution-while-the-rows-running-lengthwise-represent-the-different-strengths-to-be-used-in-the-experiment-starting-with-the-lowest-dilution-ie-the-strongest-the-cylinder-is-shaken-then-5-cc-are-measured-into-the-tubes-of-the-row-to-receive-that-strength-using-a-5-cc-delivery-pipette-in-order-to-economize-glassware-the-same-pipette-is-used-for-measuring-out-t-image235270338.htmlRMRJNDM2–. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration. STANDARDIZATION OF DISINFECTANTS 239 ning crosswise represent the same strength dilution, while the rows running lengthwise represent the different strengths to be used in the experiment. Starting with the lowest dilution (i.e., the strongest), the cylinder is shaken, then 5 cc. are measured into the tubes of the row to receive that strength, using a 5 cc. delivery pipette. In order to economize glassware, the same pipette is used for measuring out t
. Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians. Bacteriology. 32 APPLIED BACTERIOLOGY The heat must be sufficient to keep the water in vigoroas ebullition, so that the steam issues freely from the top. In this way a uniform temperature of 100° C. is maintained in the apparatus. The steriliser is fitted with a wire basket or metal rack for the reception of test-tubes containing nutrient media. This apparatus is employed for sterilising media and apparatus which cannot be exposed to temperatures higher. Fig. 3.—Steam Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/applied-bacteriology-an-introductory-handbook-for-the-use-of-students-medical-officers-of-health-analysts-and-sanitarians-bacteriology-32-applied-bacteriology-the-heat-must-be-sufficient-to-keep-the-water-in-vigoroas-ebullition-so-that-the-steam-issues-freely-from-the-top-in-this-way-a-uniform-temperature-of-100-c-is-maintained-in-the-apparatus-the-steriliser-is-fitted-with-a-wire-basket-or-metal-rack-for-the-reception-of-test-tubes-containing-nutrient-media-this-apparatus-is-employed-for-sterilising-media-and-apparatus-which-cannot-be-exposed-to-temperatures-higher-fig-3steam-image232434144.htmlRMRE483C–. Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians. Bacteriology. 32 APPLIED BACTERIOLOGY The heat must be sufficient to keep the water in vigoroas ebullition, so that the steam issues freely from the top. In this way a uniform temperature of 100° C. is maintained in the apparatus. The steriliser is fitted with a wire basket or metal rack for the reception of test-tubes containing nutrient media. This apparatus is employed for sterilising media and apparatus which cannot be exposed to temperatures higher. Fig. 3.—Steam
. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 208 BACTERIOLOGY glasses infected with the crushed tubercular masses are now laid upon serum which has been poured out into glass capsules and inspissated, the tubercular mass is smeared over the surface of the serum with a strong platinum wire, and the capsules are covered with glass plates and placed in the incubator. The colonies are perfectly formed in about three weeks, when further cultures can be made from them in test-tubes. When examined under a low power, the cultures Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/manual-of-bacteriology-for-practitioners-and-students-with-especial-references-to-practical-methods-bacteriology-208-bacteriology-glasses-infected-with-the-crushed-tubercular-masses-are-now-laid-upon-serum-which-has-been-poured-out-into-glass-capsules-and-inspissated-the-tubercular-mass-is-smeared-over-the-surface-of-the-serum-with-a-strong-platinum-wire-and-the-capsules-are-covered-with-glass-plates-and-placed-in-the-incubator-the-colonies-are-perfectly-formed-in-about-three-weeks-when-further-cultures-can-be-made-from-them-in-test-tubes-when-examined-under-a-low-power-the-cultures-image232444483.htmlRMRE4N8K–. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 208 BACTERIOLOGY glasses infected with the crushed tubercular masses are now laid upon serum which has been poured out into glass capsules and inspissated, the tubercular mass is smeared over the surface of the serum with a strong platinum wire, and the capsules are covered with glass plates and placed in the incubator. The colonies are perfectly formed in about three weeks, when further cultures can be made from them in test-tubes. When examined under a low power, the cultures
. A manual of bacteriology. Bacteriology. 62 MANUAL OF BACTERIOLOGY. closed, and the pressure begins to rise. After leaving the articles to be sterilized in the autoclave for the length of time desired, the apparatus must not be opened while the steam contained within it is still under pressure, as there may be a sudden evolution of steam upon the removal of the pressure which may blow the media out of their tubes and flasks. On the other hand, the pressure must not be allowed to drop below zero, for in this case the plugs of the tubes or flasks may be sucked in. The apparatus must, therefore, Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-manual-of-bacteriology-bacteriology-62-manual-of-bacteriology-closed-and-the-pressure-begins-to-rise-after-leaving-the-articles-to-be-sterilized-in-the-autoclave-for-the-length-of-time-desired-the-apparatus-must-not-be-opened-while-the-steam-contained-within-it-is-still-under-pressure-as-there-may-be-a-sudden-evolution-of-steam-upon-the-removal-of-the-pressure-which-may-blow-the-media-out-of-their-tubes-and-flasks-on-the-other-hand-the-pressure-must-not-be-allowed-to-drop-below-zero-for-in-this-case-the-plugs-of-the-tubes-or-flasks-may-be-sucked-in-the-apparatus-must-therefore-image232372432.htmlRMRE1DBC–. A manual of bacteriology. Bacteriology. 62 MANUAL OF BACTERIOLOGY. closed, and the pressure begins to rise. After leaving the articles to be sterilized in the autoclave for the length of time desired, the apparatus must not be opened while the steam contained within it is still under pressure, as there may be a sudden evolution of steam upon the removal of the pressure which may blow the media out of their tubes and flasks. On the other hand, the pressure must not be allowed to drop below zero, for in this case the plugs of the tubes or flasks may be sucked in. The apparatus must, therefore,
. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration; Bacteriology. STANDARDIZATION OF DISINFECTANTS 239 ning crosswise represent the same strength dilution, while the rows running lengthwise represent the different strengths to be used in the experiment. Starting with the lowest dilution (i.e., the strongest), the cylinder is shaken, then 5 cc. are measured into the tubes of the row to receive that strength, using a 5 cc. delivery pipette. In order to economize glassware, the same pipette is used for m Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-methods-in-food-and-drug-laboratories-with-an-introduction-to-micro-analytical-methods-bacteriology-food-drug-adulteration-bacteriology-standardization-of-disinfectants-239-ning-crosswise-represent-the-same-strength-dilution-while-the-rows-running-lengthwise-represent-the-different-strengths-to-be-used-in-the-experiment-starting-with-the-lowest-dilution-ie-the-strongest-the-cylinder-is-shaken-then-5-cc-are-measured-into-the-tubes-of-the-row-to-receive-that-strength-using-a-5-cc-delivery-pipette-in-order-to-economize-glassware-the-same-pipette-is-used-for-m-image235270054.htmlRMRJND9X–. Bacteriological methods in food and drug laboratories, with an introduction to micro-analytical methods. Bacteriology; Food; Drug adulteration; Bacteriology. STANDARDIZATION OF DISINFECTANTS 239 ning crosswise represent the same strength dilution, while the rows running lengthwise represent the different strengths to be used in the experiment. Starting with the lowest dilution (i.e., the strongest), the cylinder is shaken, then 5 cc. are measured into the tubes of the row to receive that strength, using a 5 cc. delivery pipette. In order to economize glassware, the same pipette is used for m
![. Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Tanner, Fred Wilbur, 1888-1957. New York, John Wiley & Sons, Inc. ; [etc. ,etc. ] Stock Photo](https://c8.alamy.com/comp/RD8WCG/bacteriology-and-mycology-of-foods-food-4l-a-fig-1-types-of-fermentation-tubes-af-browns-j5-smiths-c-smiths-without-foot-d-graves-modification-of-the-durham-fermentation-tube-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-digitally-enhanced-for-readability-coloration-and-appearance-of-these-illustrations-may-not-perfectly-resemble-the-original-work-tanner-fred-wilbur-1888-1957-new-york-john-wiley-amp-sons-inc-etc-etc-RD8WCG.jpg ". Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Tanner, Fred Wilbur, 1888-1957. New York, John Wiley & Sons, Inc. ; [etc. ,etc. ] Stock Photo")
. Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Tanner, Fred Wilbur, 1888-1957. New York, John Wiley & Sons, Inc. ; [etc. ,etc. ] Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriology-and-mycology-of-foods-food-4l-a-fig-1-types-of-fermentation-tubes-af-browns-j5-smiths-c-smiths-without-foot-d-graves-modification-of-the-durham-fermentation-tube-please-note-that-these-images-are-extracted-from-scanned-page-images-that-may-have-been-digitally-enhanced-for-readability-coloration-and-appearance-of-these-illustrations-may-not-perfectly-resemble-the-original-work-tanner-fred-wilbur-1888-1957-new-york-john-wiley-amp-sons-inc-etc-etc-image231920880.htmlRMRD8WCG–. Bacteriology and mycology of foods. Food. 4l^% a Fig 1 —Types of Fermentation Tubes. Af Brown's, J5, Smith's C, Smith's, without foot, D, Graves' Modification of the Durham Fermentation Tube. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Tanner, Fred Wilbur, 1888-1957. New York, John Wiley & Sons, Inc. ; [etc. ,etc. ]
. Bacteriological methods in food and drugs laboratories : with an introduction to micro-analytical methods . Bacteriology; Food; Drugs. STANDARDIZATION OF DISINFECTANTS 239 ning crosswise represent the same strength dilution, while the rows running lengthwise represent the different strengths to be used in the experiment. Starting with the lowest dilution {i.e., the strongest), the cyKnder is shaken, then 5 cc. are measured into the tubes of the row to receive that strength, using a 5 cc. delivery pipette. In order to economize glassware, the same pipette is used for measuring out the next di Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/bacteriological-methods-in-food-and-drugs-laboratories-with-an-introduction-to-micro-analytical-methods-bacteriology-food-drugs-standardization-of-disinfectants-239-ning-crosswise-represent-the-same-strength-dilution-while-the-rows-running-lengthwise-represent-the-different-strengths-to-be-used-in-the-experiment-starting-with-the-lowest-dilution-ie-the-strongest-the-cyknder-is-shaken-then-5-cc-are-measured-into-the-tubes-of-the-row-to-receive-that-strength-using-a-5-cc-delivery-pipette-in-order-to-economize-glassware-the-same-pipette-is-used-for-measuring-out-the-next-di-image232350738.htmlRMRE0DMJ–. Bacteriological methods in food and drugs laboratories : with an introduction to micro-analytical methods . Bacteriology; Food; Drugs. STANDARDIZATION OF DISINFECTANTS 239 ning crosswise represent the same strength dilution, while the rows running lengthwise represent the different strengths to be used in the experiment. Starting with the lowest dilution {i.e., the strongest), the cyKnder is shaken, then 5 cc. are measured into the tubes of the row to receive that strength, using a 5 cc. delivery pipette. In order to economize glassware, the same pipette is used for measuring out the next di
. Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians. Bacteriology. 68 APPLIED BACTERIOLOGY subsequently make from it. It may so happen that the dilution may in some cases have been carried too far, in which event we shall obtain the plate we require from the second tube; but success in this operation is a matter' of experience and judgment. When inoculating the tubes, care must be taken to hold the plug of cotton-wool between the fingers, best between the third and fourth, using the back of the hand, and thus twist it o Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/applied-bacteriology-an-introductory-handbook-for-the-use-of-students-medical-officers-of-health-analysts-and-sanitarians-bacteriology-68-applied-bacteriology-subsequently-make-from-it-it-may-so-happen-that-the-dilution-may-in-some-cases-have-been-carried-too-far-in-which-event-we-shall-obtain-the-plate-we-require-from-the-second-tube-but-success-in-this-operation-is-a-matter-of-experience-and-judgment-when-inoculating-the-tubes-care-must-be-taken-to-hold-the-plug-of-cotton-wool-between-the-fingers-best-between-the-third-and-fourth-using-the-back-of-the-hand-and-thus-twist-it-o-image232434106.htmlRMRE4822–. Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians. Bacteriology. 68 APPLIED BACTERIOLOGY subsequently make from it. It may so happen that the dilution may in some cases have been carried too far, in which event we shall obtain the plate we require from the second tube; but success in this operation is a matter' of experience and judgment. When inoculating the tubes, care must be taken to hold the plug of cotton-wool between the fingers, best between the third and fourth, using the back of the hand, and thus twist it o
. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 32 BACTERIOLOGY a centrifugal machine, or centrifuge, to be driven by hand. This contrivance carries a metal frame or a disc with several apertures in which are fixed metal cases for the reception of small glass tubes. The fluid to be examined is poured. Fig. 20.—Stesbeok's Centrifuge (after Jakscli). into the small tubes, which are provided at their lower end with a little reservoir communicating with them through a conical constriction, and in which the precipitate gathers wh Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/manual-of-bacteriology-for-practitioners-and-students-with-especial-references-to-practical-methods-bacteriology-32-bacteriology-a-centrifugal-machine-or-centrifuge-to-be-driven-by-hand-this-contrivance-carries-a-metal-frame-or-a-disc-with-several-apertures-in-which-are-fixed-metal-cases-for-the-reception-of-small-glass-tubes-the-fluid-to-be-examined-is-poured-fig-20stesbeoks-centrifuge-after-jakscli-into-the-small-tubes-which-are-provided-at-their-lower-end-with-a-little-reservoir-communicating-with-them-through-a-conical-constriction-and-in-which-the-precipitate-gathers-wh-image232435312.htmlRMRE49H4–. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 32 BACTERIOLOGY a centrifugal machine, or centrifuge, to be driven by hand. This contrivance carries a metal frame or a disc with several apertures in which are fixed metal cases for the reception of small glass tubes. The fluid to be examined is poured. Fig. 20.—Stesbeok's Centrifuge (after Jakscli). into the small tubes, which are provided at their lower end with a little reservoir communicating with them through a conical constriction, and in which the precipitate gathers wh
. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. FILLING THB TUBES 135 and by pressing the pinchcock with which the funnel is provided the desired amount of material (5-10 c.c.) may be allowed to flow into the tubes held under its opening. It is not necessary to sterilize the funnel', for the medium is to be subjected to this process as soon as it is in the test- tubes. Fig. 22. Funnel for filling tubes with culture-media. Care should be taken that none of the medium is dropped upon the mouth of the test-tube, otherwise the cotton plug becomes adh Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/the-principles-of-bacteriology-a-practical-manual-for-students-and-physicians-bacteriology-filling-thb-tubes-135-and-by-pressing-the-pinchcock-with-which-the-funnel-is-provided-the-desired-amount-of-material-5-10-cc-may-be-allowed-to-flow-into-the-tubes-held-under-its-opening-it-is-not-necessary-to-sterilize-the-funnel-for-the-medium-is-to-be-subjected-to-this-process-as-soon-as-it-is-in-the-test-tubes-fig-22-funnel-for-filling-tubes-with-culture-media-care-should-be-taken-that-none-of-the-medium-is-dropped-upon-the-mouth-of-the-test-tube-otherwise-the-cotton-plug-becomes-adh-image232442323.htmlRMRE4JFF–. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. FILLING THB TUBES 135 and by pressing the pinchcock with which the funnel is provided the desired amount of material (5-10 c.c.) may be allowed to flow into the tubes held under its opening. It is not necessary to sterilize the funnel', for the medium is to be subjected to this process as soon as it is in the test- tubes. Fig. 22. Funnel for filling tubes with culture-media. Care should be taken that none of the medium is dropped upon the mouth of the test-tube, otherwise the cotton plug becomes adh
. Laboratory work in bacteriology. Bacteriology. BLOOD-SERUM. 467 thermo-regulator is employed to secure a constant temperature. Heat is applied till a temperature of about 75° is reached and this is then maintained for 1 hour. This is repeated on each of three or four successive days.. The time necessary to secure sterilization can be shortened by transferring- the tubes, for a few hours after each heat, to an incubator at 37° as described in connection with the preceding method. Sterilization of serum at 100°.—Serum sterilized at this temperature is opaque white. This, howevei:, does not in- Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/laboratory-work-in-bacteriology-bacteriology-blood-serum-467-thermo-regulator-is-employed-to-secure-a-constant-temperature-heat-is-applied-till-a-temperature-of-about-75-is-reached-and-this-is-then-maintained-for-1-hour-this-is-repeated-on-each-of-three-or-four-successive-days-the-time-necessary-to-secure-sterilization-can-be-shortened-by-transferring-the-tubes-for-a-few-hours-after-each-heat-to-an-incubator-at-37-as-described-in-connection-with-the-preceding-method-sterilization-of-serum-at-100serum-sterilized-at-this-temperature-is-opaque-white-this-howevei-does-not-in-image232076944.htmlRMRDG0E8–. Laboratory work in bacteriology. Bacteriology. BLOOD-SERUM. 467 thermo-regulator is employed to secure a constant temperature. Heat is applied till a temperature of about 75° is reached and this is then maintained for 1 hour. This is repeated on each of three or four successive days.. The time necessary to secure sterilization can be shortened by transferring- the tubes, for a few hours after each heat, to an incubator at 37° as described in connection with the preceding method. Sterilization of serum at 100°.—Serum sterilized at this temperature is opaque white. This, howevei:, does not in-
![. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. METHODS USED IN CULTIVATION OF BACTERIA 145 The plates when fitted together are sterilized and thus form a closed cell which, if properly handled, may remain sterile indefinitely. The technique for maJdrtg a pour plate for the purpose of isolating bacteria from mixed culture is as follows: The actual "pouring" of plates is preceded by the preparation of usually three graded dilutions of the material to be examined. For this purpose three agar or gelatin tubes are melted and, Stock Photo](https://c8.alamy.com/comp/RE4720/a-text-book-of-bacteriology-a-practical-treatise-for-students-and-practitioners-of-medicine-bacteriology-methods-used-in-cultivation-of-bacteria-145-the-plates-when-fitted-together-are-sterilized-and-thus-form-a-closed-cell-which-if-properly-handled-may-remain-sterile-indefinitely-the-technique-for-majdrtg-a-pour-plate-for-the-purpose-of-isolating-bacteria-from-mixed-culture-is-as-follows-the-actual-quotpouringquot-of-plates-is-preceded-by-the-preparation-of-usually-three-graded-dilutions-of-the-material-to-be-examined-for-this-purpose-three-agar-or-gelatin-tubes-are-melted-and-RE4720.jpg ". A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. METHODS USED IN CULTIVATION OF BACTERIA 145 The plates when fitted together are sterilized and thus form a closed cell which, if properly handled, may remain sterile indefinitely. The technique for maJdrtg a pour plate for the purpose of isolating bacteria from mixed culture is as follows: The actual "pouring" of plates is preceded by the preparation of usually three graded dilutions of the material to be examined. For this purpose three agar or gelatin tubes are melted and, Stock Photo")
. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. METHODS USED IN CULTIVATION OF BACTERIA 145 The plates when fitted together are sterilized and thus form a closed cell which, if properly handled, may remain sterile indefinitely. The technique for maJdrtg a pour plate for the purpose of isolating bacteria from mixed culture is as follows: The actual "pouring" of plates is preceded by the preparation of usually three graded dilutions of the material to be examined. For this purpose three agar or gelatin tubes are melted and, Stock Photohttps://www.alamy.com/licenses-and-pricing/?v=1https://www.alamy.com/a-text-book-of-bacteriology-a-practical-treatise-for-students-and-practitioners-of-medicine-bacteriology-methods-used-in-cultivation-of-bacteria-145-the-plates-when-fitted-together-are-sterilized-and-thus-form-a-closed-cell-which-if-properly-handled-may-remain-sterile-indefinitely-the-technique-for-majdrtg-a-pour-plate-for-the-purpose-of-isolating-bacteria-from-mixed-culture-is-as-follows-the-actual-quotpouringquot-of-plates-is-preceded-by-the-preparation-of-usually-three-graded-dilutions-of-the-material-to-be-examined-for-this-purpose-three-agar-or-gelatin-tubes-are-melted-and-image232433320.htmlRMRE4720–. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. METHODS USED IN CULTIVATION OF BACTERIA 145 The plates when fitted together are sterilized and thus form a closed cell which, if properly handled, may remain sterile indefinitely. The technique for maJdrtg a pour plate for the purpose of isolating bacteria from mixed culture is as follows: The actual "pouring" of plates is preceded by the preparation of usually three graded dilutions of the material to be examined. For this purpose three agar or gelatin tubes are melted and,
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