. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. PROTEIN DIFFERANCES 179 Cortex Nucleu: jVv A B D F. t^jrwLMVf. WOi .w»-J ^"iVn. , -.--1 p«,» -M^ FIGURE 1. Electrophoretic patterns of protein from the cortex and nucleus of channel rock- fish lenses that were: fresh (A); frozen once (B); frozen, thawed, and refrozen (C); left in a decomposing fish head in noncirculating sea water at room tem- perature for five days (D); stored outdoors in the shade for five days (E); and store
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. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. PROTEIN DIFFERANCES 179 Cortex Nucleu: jVv A B D F. t^jrwLMVf. WOi .w»-J ^"iVn. , -.--1 p«, » -M^ FIGURE 1. Electrophoretic patterns of protein from the cortex and nucleus of channel rock- fish lenses that were: fresh (A); frozen once (B); frozen, thawed, and refrozen (C); left in a decomposing fish head in noncirculating sea water at room tem- perature for five days (D); stored outdoors in the shade for five days (E); and stored outdoors in the sun for five days (F). The dashed line indicates the zone of sample application, to the left of which is the cathode. duced by the more superficial nuclear lens layers are more intensely stained overall, but in all patterns of nuclear lens, the farthest-migrat- ing fraction stained the darkest. In both experiments, the electrophoretic patterns demonstrated a predominantly cathodal migration of lens proteins. However, in elec- trophoretic patterns of cortical lens protein from another species, the Pacific albacore, TJiuvniis ahiJunga, a discrete anodal-migrating protein was noted (Smith, 1966). This protein could be serum albumin, since this molecule has a highly electronegative charge; readily traverses the lens capsule (Orzalesi and Pirodda, 1955) ; and might normally be in eye humor, as are some other serum proteins (Utter and liodgins, 1969). SUMMARY AND CONCLUSIONS Albumins and globulins were extracted from the aqueous humor and the lens cortices and nuclei of individual channel rockfish. Protein from the nuclei, where staining indicated that it is concentrated in tlie outer layers, was more resistant to denaturation and produced a simpler electrophoretic pattern than cortical protein.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustra