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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. TYROSINASE AND PUPAL RESPIRATION RESULTS Experiments with intact pupae The respiration of the intact animal was studied by determining the effect of the injection of the following kinds of compounds: 1. Phenolic substrates for tyrosinase 2. Inhibitors of tyrosinase Before the effect of the injection of these substances could be measured, it was necessary to learn to what extent the injection technique itself would affect the animal's respiration. With this in mind, varying amounts of distilled water and of insect Ringer's so

. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. TYROSINASE AND PUPAL RESPIRATION RESULTS Experiments with intact pupae The respiration of the intact animal was studied by determining the effect of the injection of the following kinds of compounds: 1. Phenolic substrates for tyrosinase 2. Inhibitors of tyrosinase Before the effect of the injection of these substances could be measured, it was necessary to learn to what extent the injection technique itself would affect the animal's respiration. With this in mind, varying amounts of distilled water and of insect Ringer's so Stock Photo
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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. TYROSINASE AND PUPAL RESPIRATION RESULTS Experiments with intact pupae The respiration of the intact animal was studied by determining the effect of the injection of the following kinds of compounds: 1. Phenolic substrates for tyrosinase 2. Inhibitors of tyrosinase Before the effect of the injection of these substances could be measured, it was necessary to learn to what extent the injection technique itself would affect the animal's respiration. With this in mind, varying amounts of distilled water and of insect Ringer's solution were introduced into the animal, after which its oxygen uptake was measured. Figure 2 and Table I show the results of the injection of 70 60 50 MM 0> PER GM. o JO , 20 I 0 O .05CC DISTILLED WATER • .05CC RINGERS SOLUTION SUBSTANCES INJECTED. IIT T I I I I I I I I I 10 2jO 30 40 50 60 70 80 90 100 NO 120 130 TIME (MINUTES) FIGURE 2. Effect of injection of distilled water and Ringer's solution on the oxygen uptake of pupae of P. cecropia. .05 cc. distilled water and .1 cc. of insect Ringer's solution into the animal. It is apparent from these results that a rise in Qo2 occurs upon the injection into the animal of either distilled water or insect Ringer's solution. This increase was found to occur whenever the integument of the animal was broken upon the inser- tion of the syringe needle, even if nothing was injected, so that it was necessary to include this factor in calculating the results of the subsequent injection experiments. That this increase in respiration was not due merely to increased movements of the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Fr