The Biochemical journal, 1907 . Fig. II.—Frogs gastrocnemius, dead ten days.. Fig. 12.—Frogs stomach, dead three days. In the three groups of vertebrates, then, we obtain in rigoredmuscle only one heat contraction, due to effects upon connectivetissue elements. That previous observers have not noted this in thecase of frogs muscle is probably due to their having underestimatedthe lapse of time necessary to bring on rigor mortis in this animal.In many instances we have obtained the first contraction when theanimal has been lying in saline solution apparently dead for several days,but in all the

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The Biochemical journal, 1907 . Fig. II.—Frogs gastrocnemius, dead ten days.. Fig. 12.—Frogs stomach, dead three days. In the three groups of vertebrates, then, we obtain in rigoredmuscle only one heat contraction, due to effects upon connectivetissue elements. That previous observers have not noted this in thecase of frogs muscle is probably due to their having underestimatedthe lapse of time necessary to bring on rigor mortis in this animal.In many instances we have obtained the first contraction when theanimal has been lying in saline solution apparently dead for several days, but in all these cases, on testing the matter, we have found that themuscles would respond to an electrical stimulus. If still further time HEAT RIGOR IN VERTEBRATE MUSCLE 375 be allowed to elapse, so that no reaction will occur on stimulation, then the muscles will give only one heat contraction, viz., that dueto connective tissue at 52° C. Further, if the animals are killed bychloroform, rapid rigor, as is well known, will ensue, and in themuscles from an animal so killed we get onl